›› 2012, Vol. 32 ›› Issue (8): 1020-.doi: 10.3969/j.issn.1674-8115.2012.08.013

• Monographic report (Infertility and assisted reproductive technology) • Previous Articles     Next Articles

A new technique of mouse intracytoplasmic sperm injection without assistance of Piezo for high survival rate of oocytes

MIAO Cong-xiu1, YAN Zhi-guang2, YANG Hong-mei1, LIANG Hong-xing2, YU Sha3, KUANG Yan-ping4, LV Qi-feng4   

  1. 1.Heping Hospital of Changzhi Medical College, Changzhi 046000, China；2.State Key Laboratory for Conservation and Utilization of Subtropical AgroBioresources, Animal Reproduction Institute, Guangxi University, Nanning 530004, China；3.Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 200021, China|4.Department of Reproductive Medicine, the Ninth People´s Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200011, China
  • Online:2012-08-28 Published:2012-08-29
  • Supported by:

    National Nature Science Foundation of China, 31071275；Shanghai Science and Technology Committee Foundation, 09411962900, 11411950105

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Abstract:

Objective To achieve higher survival rate of mouse oocytes through modification of established technique of mouse intracytoplasmic sperm injection (ICSI) without assistance of Piezo. Methods The step of deeply sucking and deeply insertion to mouse oocytes in the established method (one-step method without Piezo-ICSI group) was modified to two steps (two-step method without Piezo-ICSI group), in which slightly sucking and slightly insertion was first performed before deeply sucking and deeply insertion. Besides, oocytes of parthenogenetic activation were served as control group. The survival rate, fertilization rate and cleavage rate of oocytes were compared between one-step method without Piezo-ICSI group and two-step method without Piezo-ICSI group. Results The survival rate of oocytes in two-step method without Piezo-ICSI group was significantly higher than that in one-step method without Piezo-ICSI group (92.2% vs 83.7%, P<0.01), while there was no significant difference in both fertilization rate and cleavage rate of oocytes between two group (P>0.05). Conclusion Some oolemma becomes fragile when the shape of the oocyte membrane is changed by drawing deeply with new type of holding pipette. The new technique of mouse ICSI avoids this sensitive fragile by two-step method for oocyte holding and insertion, which is beneficial to achieve higher survival rate of mouse oocytes.

Key words: intracytoplasmic sperm injection, oocytes, oocyte membrane, puncture, survival rate, mouse