• Original article (Clinical research) • Previous Articles     Next Articles

Effect of circulating miR-192 on early diagnosis of acute kidney injury after cardiac surgery

XU Yuan, ZHANG Lu-lu, YAN Yu-cheng, XUE Song, SHAO Xing-hua, GUAN Xue-jing, ZHU Ming-li, DAI Hui-li   

  1. Renal Division, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200127, China
  • Online:2015-06-28 Published:2015-07-30
  • Supported by:

    National Natural Scientific Foundation of China, 81170687， 81470918; Foundation of Science and Technology Commission of Shanghai Municipality, 134119a2300， 12140904000

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Abstract:

Objective To investigate the value of circulating miR-192 in early diagnosis of acute kidney injury (AKI)  after cardiac surgery. Methods Blood samples were prospectively collected at different time points before and after cardiac surgery. After cardiac surgery, serum creatinine (Scr) and miR-192 levels of 35 patients with AKI and 35 patients without AKI whose clinical data were similar to patients with AKI were detected. Dynamic changes of miR-192 and Scr levels of two groups during the perioperative period were observed.  Receiver operating characteristic (ROC) curves and areas under curves (AUC) were used to evaluate the precision of diagnosing AKI by miR-192. AKI was defined as the increase of Scr level was above 0.3mg/dL or 1.5 times of baseline value within 48 h after surgery. Results The  mean age of 70 patients was (64.31±8.17) and the baseline Scr level was (74.97±16.25) μmol/L. The median time of diagnosing AKI by Scr of the AKI group was 24 h after admission in ICU. There was no significant difference in the level of plasma miR-192 between two groups before surgery. Compared with levels of plasma miR-192 of two groups before surgery, levels of plasma miR-192 after surgery immediately increased [1.12 (0.47, 2.42) vs 1.93 (0.93, 3.24) and 0.79 (0.41,1.16) vs 1.65 (0.97, 2.36) for patients with AKI and without AKI, P<0.05]. Levels of plasma miR-192 of the AKI group were higher, but the difference of two groups was not statistically significant. The level of plasma miR-192 of the AKI group 2 h after surgery continuously increased, while that of the non-AKI group began to decrease. The difference of two groups was statistically significant [1.80 (0.94, 5.10) vs 1.21 (0.88, 1.77), P<0.05]. AUC for diagnosing AKI by plasma miR-192 was 0.67 (95%CI 0.54-0.81, P=0.01). Conclusion The level of plasma miR-192 of patients with AKI significantly increases at early stage after cardiac surgery and the level of circulating miR-192 2 h after surgery can be used as a biomarker for early diagnosis of AKI after cardiac surgery.

Key words: acute kidney injury, cardiac surgery, miR-192, biomarker