• Original article (Basic research) • Previous Articles     Next Articles

Pro-1 regulates the lifespan of Caenorhabditis elegans through DAF-16

MAO Yu-qin1, HAN San-feng2, WANG Li-shun1   

  1. 1.Center for Molecular Medicine, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China; 2.Minhang Hospital, Fudan University, Shanghai 201100, China
  • Online:2016-10-28 Published:2016-11-29
  • Supported by:

    National Natural Science Foundation of China, 81472758, 31170783

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Abstract:

Objective·To observe the effects of pro-1 gene on the lifespan of Caenorhabditis elegans (C. elegans) and preliminary explore the molecular mechanisms. Methods·RNAi technique was used to intervene the expression of pro-1 gene in C. elegans. The lifespan of C. elegans with silent pro-1 was analyzed and changes in growth, development, motion, diet, and reproduction were detected. The molecular mechanisms of pro-1 to affect the lifespan were explored using RNAi technique, fluorescence localization technology, and RT-PCR. Results·The lifespan of silence group C. elegans was significantly increased by 14.58% after the expression of pro-1  intervented as compared with controls (P=0.002). DAF-16 translocated into nucleus in C. elegans  of TJ356 strain after intervening the expression of pro-1 and mRNA expressions of downstream target genes such as old-1, sod-3, hsp-16.1, mtl-1 and ctl-2 were up-regulated. Conclusion·pro-1 prolongs the lifespan of C. elegans via promoting the translocation of DAF-16 into nucleus and up-regulating the mRNA expressions of downstream target genes.

Key words: Caenorhabditis elegans, pro-1, daf-16, lifespan