Establishment of continuous glucose monitoring in mice and multiscale entropy analysis of glucose time series

doi:10.3969/j.issn.1674-8115.2021.02.002

JOURNAL OF SHANGHAI JIAOTONG UNIVERSITY (MEDICAL SCIENCE) ›› 2021, Vol. 41 ›› Issue (2): 134-139.doi: 10.3969/j.issn.1674-8115.2021.02.002

• Basic research • Previous Articles Next Articles

Establishment of continuous glucose monitoring in mice and multiscale entropy analysis of glucose time series

Cheng LI¹(), Ming-liang ZHANG¹, Ling-wen YING¹, Jiao-rong SU¹, Rui TAO², Xia YU², Yu-qian BAO¹, Jian ZHOU¹()

^1.Department of Endocrinology and Metabolism, Shanghai Sixth People's Hospital, Shanghai Jiao Tong University; Shanghai Diabetes Institute; Shanghai Key Laboratory of Diabetes Mellitus; Shanghai Clinical Center for Diabetes, Shanghai 200233, China
^2.College of Information Science and Engineering, Northeastern University, Shenyang 110819, China

Received:2020-04-22 Online:2021-02-28 Published:2021-02-28
Contact: Jian ZHOU E-mail:licheng_sjtu@sjtu.edu.cn;zhoujian@sjtu.edu.cn
Supported by:
National Key Research and Development Program of China(2018YFC2001004);National Natural Science Foundation of China(61903071);Shanghai Municipal Education Commission—Gaofeng Clinical Medicine Grant Support(20161430)

Abstract

Abstract:

Objective·To establish continuous glucose monitoring (CGM) in mice and implement multiscale entropy (MSE) analysis of glucose time series. Methods·Diet-induced obesity (DIO) mice (n=3) and control mice (n=3) were selected as the research objects. The blood glucose and body temperature data of the two groups were collected by using the implantable glucose telemetry system. The data of 10?14 days after operation were analyzed, and the recording time of the system was counted. Using MATLAB R2019b software, MSE analysis was performed on glucose time series of the two groups from 11 to 17 days after operation, and the corresponding entropy value on each time scale was calculated. Results·The CGM technology based on the implantable glucose telemetry system in mice was successfully established. The average recording time of the 6 mice was (27.3±9.3) d, and 232 887 blood glucose values were obtained. The mean blood glucose level of the DIO mice was (7.04±0.71) mmol/L and the mean body temperature was (33.34±0.18) ℃. Compared with that of the control mice, the glucose time series complexity of the DIO mice was lower, and the difference between the two groups was not statistically significant. Conclusion·The CGM technology in mice is successfully established. MSE analysis shows that the complexity of glucose time series in the DIO mice decreases, which may be one of the manifestations of abnormal glucose metabolism in the early stage.

Key words: continuous glucose monitoring (CGM), implantable glucose telemetry system, multiscale entropy (MSE), complexity, time series, diet-induced obesity mouse

CLC Number:

R589.9

Cheng LI, Ming-liang ZHANG, Ling-wen YING, Jiao-rong SU, Rui TAO, Xia YU, Yu-qian BAO, Jian ZHOU. Establishment of continuous glucose monitoring in mice and multiscale entropy analysis of glucose time series[J]. JOURNAL OF SHANGHAI JIAOTONG UNIVERSITY (MEDICAL SCIENCE), 2021, 41(2): 134-139.

Figures/Tables 6

Fig 1 Operation pattern of HD-XG glucose telemetry implant in mice

Tab 1 Recording time and the number of glucose values of the two groups monitored by implantable glucose telemetry system

Index	DIO group (N=3)			Control group (N=3)
Index	1	2	3	1	2	3
Recording time/d	20	39	39	18	24	24
Total glucose number	27 192	55 906	55 799	25 007	34 455	34 528

Tab 2 Mean blood glucose and body temperature of each mouse in the two groups from Day 10 to Day 14

Index	DIO group (N=3)			Control group (N=3)
Index	1	2	3	1	2	3
Mean blood glucose/(mmol·L^-1)	6.74±0.62	6.54±0.93	7.86±0.81	6.96±1.68	7.38±1.05	6.42±1.08
Mean body temperature/℃	33.15±1.77	33.37±0.61	33.50±0.74	33.02±0.93	34.29±0.84	34.06±0.89

Fig 2 Glucose profiles of mice in the two groups from Day 10 to Day 14

Fig 3 Body temperature profiles of mice in the two groups from Day 10 to Day 14

Fig 4 Entropy of glucose time series on different scales in the two groups

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Establishment of continuous glucose monitoring in mice and multiscale entropy analysis of glucose time series

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