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Diagnostic value of ELISPOT by recombinant Rv0315 protein of Mycobacterium tuberculosis in detection of active pulmonary tuberculosis

ZHOU Ye-cheng1, CHEN Cui-cui2, YE Juan3, ZHAO Jun-wei4, XUAN Song-hua3, YANG Zhi-rong1, ZHANG Shu-lin4   

  1. 1.College of Life Sciences, Sichuan University, Chengdu 610065, China; 2.The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450001, China; 3.Department of Biochemistry, Henan University of Traditional Chinese Medicine, Zhengzhou 450008, China； 4.Department of Medical Microbiology and Parasitology, Basic Medical College, Shanghai Jiaotong University, Shanghai 200025, China
  • Online:2013-07-28 Published:2013-08-22
  • Supported by:

    Shanghai Science and Technology Committee Foundation, 12441903300; National Natural Science Foundation of China, 81271794; National Major Science and Technology Research Project in the “12th Five-year Plan”, 2013ZX10003002-005

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Abstract:

Objective To investigate the application of enzyme-linked immunospot assay (ELISPOT) by recombinant Rv0315 protein of Mycobacterium tuberculosis in the auxiliary diagnosis of active pulmonary tuberculosis. Methods A total of 25 patients with initial pulmonary tuberculosis (tuberculosis group) and 28 healthy people (control group) were enrolled. The peripheral blood mononuclear cells of all participants were co-cultured with Rv0315, earlier secreted antigen target 6 (EAST6) and culture filtrate protein 10 (CFP10) antigens, and spot forming cells (SFCs) were enumerated by ELISPOT. Results The values of sensitivity of Rv0315, EAST6 and CFP10 antigens by ELISPOT were 84.0% (21/25), 80.0% (20/25) and 92.0% (23/25) respectively, and those of specificity were 89.2% (25/28), 89.2% (25/28) and 92.8% (26/28) respectively. Rv0315 was more sensitive than EAST6, but was less sensitive in comparison with CFP10 in diagnosis of Mycobacterium tuberculosis infection. Conclusion ELISPOT with recombinant Rv0315 protein as antigen has the potential value in the auxiliary diagnosis of active pulmonary tuberculosis.

Key words: Mycobacterium tuberculosis, Rv0315, enzyme-linked immunospot assay, cellular immunity