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Effects of CTRP3 on TGF-β1 induced proliferation and α-SMA expression in adventitial fibroblasts

LIN Shao-hui, SHENG Jing, MA Shao-jun, LU Ping, CAI Wen-wei, HU Ping   

  1. Department of Geriatrics, the Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China
  • Online:2014-03-28 Published:2014-04-02
  • Supported by:

    Foundation of Science and Technology Commission of Shanghai Municipality, 10JC1408902

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Abstract:

Objective To investigate the effects of C1q/TNF-related protein-3 (CTRP3) on transforming growth factor-β1 (TGF-β1) induced proliferation and expression of α-smooth muscle actin (α-SMA) among adventitial fibroblasts (AFs). Methods AFs were isolated from thoracic aortas of male Sprague Dawly (SD) rats. The cells in passage 3 to 5 were treated with various concentrations of CTRP3 (0.1, 1, 10, 50 μg/mL). The effects of CTRP3 on proliferation of AFs were examined by Cell Counting Kit (CCK-8). Immunoflueresence assay, Real-Time PCR, and Western blotting were adopted to detect the expression of α-SMA of AFs. Results CTRP3 attenuated the proliferative activities induced by TGF-β1 in a dose-dependent manner, and the most marked effect could be observed at the concentration of 10 μg/mL (P<0.01). Immunoflueresence assay, Real-Time PCR and Western blotting showed that CTRP3 could significantly inhibited TGF-β1 induced upregulation of α-SMA both at the mRNA and protein levels (P<0.01). Conclusion CTRP3 has significant inhibition effects on proliferation and α-SMA expression of adventitial fibroblasts induced by TGF-β1, which suggests the potential value of CTRP3 in the treatment of pathological vascular remodeling.

Key words: adventitial fibroblasts, C1q/TNF-related protein-3, transforming growth factor-β1, α-smooth muscle actin, proliferation