Abstract: Objective · To study the of liver-specific ZP domain-containing protein (LZP) in momodels of obesity. Methods · The gene and protein of LZP in different tissues of C57BL/6J mice were detectedrealtime-PCR and Western blotting respectively. C57BL/6J mice were treated with high fat diet (HFD) to establish the model of diet-induced obesity and ob/ob mice were also treated with HFD. The body mass and blood glucose were monitored during the experiment, then the liver weight and fat mass were measured at the end of the study. Hematoxylin-eosin staining of liver was performed to observe the morphology of liver. The of LZP in liver of model mice was also detectedrealtime-PCR and Western blotting, respectively. Results · The of LZP mRNA was mainly found in liver, while a lower gene level was also observed in several other tissues such as spleen and testisrealtime-PCR. The protein of LZP was detected in liver in C57BL/6J miceWestern blotting. Compared with normal diet group, the group treated with HFD had significantly increased body mass and total fat mass, higher blood glucose, increased liver mass and more serious hepatic steatosis (all P<0.05), while the of LZP in liver was reduced (P<0.05). Similarly, body mass and blood glucose were increased significantly in ob/ob mice (both P<0.05), though the of LZP was decreased compared with wild type littermates (P<0.05). Conclusion · Momodels of obesity display decreased of LZP in liver, indicating that LZP may play a role in metabolic homeostasis in obese individuals.

Key words: liver-specific ZP domain-containing protein, obesity, hepatokine