JOURNAL OF SHANGHAI JIAOTONG UNIVERSITY (MEDICAL SCIENCE) ›› 2020, Vol. 40 ›› Issue (11): 1473-1476.doi: 10.3969/j.issn.1674-8115.2020.11.006

• Original article (Basic research) • Previous Articles     Next Articles

Determination of half-life and half-lethal dose of itaconic acid in C57BL/6J male mice

GUO Yang-yang1, 2, LEI He-hua2, WANG Yu-lan3   

  1. 1. University of Chinese Academy of Sciences, Beijing 100049, China; 2. State Key Laboratory of Spectroscopy and Atomic and Molecular Physics, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan Magnetic Resonance Center, Wuhan 430071, China; 3. Singapore Phenome Center, Lee Kong Chian School of Medicine, School of Biological Sciences, Nanyang Technological University, Singapore 639646, Singapore
  • Online:2020-11-28 Published:2021-01-13
  • Supported by:
    National Key Research and Development Program (2017YFC0906800).

Abstract: Objective · To determine the half-life and half-lethal dose of itaconic acid in mice, and provide intuitive data for the metabolic rate and toxicity of itaconic acid in mice. Methods · Itaconic acid was injected into mice through caudal vein. The concentration of itaconic acid was measured by blood collection under fundus venous plexus at different time. At the same time, the survival of the mice was recorded after injection for 14 d. Results · Under the conditions of this experiment, the half-life of itaconic acid in male C57BL/6J mice was 29.13 min, and the half-lethal dose of is 258.263 mg/kg. Conclusion · Itaconic acid has a fast metabolic rate and a short half-life in mice. Itaconic acid is less toxic, based on its half-lethal dose in mice.

Key words: itaconic acid, mouse, half-life, half-lethal dose

CLC Number: