Effect of transient receptor potential vanilloid 4 on traumatic heterotopic ossification around temporomandibular joint

doi:10.3969/j.issn.1674-8115.2022.12.001

Abstract

Abstract:

Objective ·To investigate the effect of transient receptor potential vanilloid 4 (TRPV4) on traumatic heterotopic ossification around temporomandibular joint (THO-TMJ). Methods ·Eighteen eight-week-old male mice were divided into two groups by simple random sampling. Half right condylar cartilage was removed in every mouse. Nine mice in experimental group was administered TRPV4 blocker [14 mg/(kg·d)] via oral gavage. The same amount of saline was given to nine mice in control group. The skull samples were collected at 14, 30 and 90 d after surgery. The heterotopic ossification was evaluated by H-E staining and Micro CT scan. Three-dimensional reconstruction of the skulls was performed to measure the volume and surface area of condyles. Morphometric index (MI) was applied to indicate the degree of heterotopic ossification in both groups. Results ·H-E staining results showed that active endochondral ossification occurred around the injured condyles in both groups. Micro CT images showed that the tissue density around the condyles in both groups gradually increased in the TMJ area after operation, and then the ectopic bone tissues gradually proliferated outside the condyle, partially connecting to the joint fossa. Scattered osteophytes were seen in the joint space. The trend of TMJ bone fusion was more obvious in the control group. The morphological index of the experimental group (MI=0.15±0.01) was lower than that of the control group (MI=0.18±0.03, P<0.05). Conclusion ·Blocking TRPV4 function can help reduce the formation and progression of THO-TMJ.

Key words: temporomandibular joint, traumatic heterotopic ossification, TRPV4

CLC Number:

R782.6

ZHU Yue, GU Jiaying, DAI Jiewen. Effect of transient receptor potential vanilloid 4 on traumatic heterotopic ossification around temporomandibular joint[J]. Journal of Shanghai Jiao Tong University (Medical Science), 2022, 42(12): 1649-1655.

Figures/Tables 3

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References 35

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