Journal of Shanghai Jiao Tong University (Medical Science) ›› 2025, Vol. 45 ›› Issue (9): 1106-1115.doi: 10.3969/j.issn.1674-8115.2025.09.003

• Basic research • Previous Articles     Next Articles

Effect of jaw osteoblasts on B cell development via cytokine secretion

WANG Xinyu1, CHEN Qianye2, SUN Jiping3, LU Tingwei1, HUANG Xiangru1, SUN Siyuan1, LIU Yuanqi1, PAN Houwen1, DAI Qinggang4, SHEN Lei3, JIANG Lingyong1()   

  1. 1.Department of Oral and Maxillofacial Surgery, Shanghai Ninth People′s Hospital, Shanghai Jiao Tong University School of Medicine; College of Stomatology, Shanghai Jiao Tong University; National Center for Stomatology; National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology; Shanghai Research Institute of Stomatology, Shanghai 200011, China
    2.Department of Oral Maxillofacio-Head and Neck Oncology, Shanghai Ninth People′s Hospital, Shanghai Jiao Tong University School of Medicine; College of Stomatology, Shanghai Jiao Tong University; National Center for Stomatology; National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology; Shanghai Research Institute of Stomatology, Shanghai 200011, China
    3.Department of Immunology and Microbiology, Shanghai Jiao Tong University School of Basic Medical Sciences; Shanghai Institute of Immunology, Shanghai 200025, China
    4.Department of 2nd Dental Centre, Shanghai Ninth People′s Hospital, Shanghai Jiao Tong University School of Medicine; College of Stomatology, Shanghai Jiao Tong University; National Center for Stomatology; National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology; Shanghai Research Institute of Stomatology, Shanghai 200011, China
  • Received:2025-02-19 Accepted:2025-04-29 Online:2025-09-28 Published:2025-09-30
  • Contact: JIANG Lingyong E-mail:jianglingyong@sjtu.edu.cn
  • Supported by:
    “Two-Hundred Talents” Program of Shanghai Jiao Tong University School of Medicine(20221809);National Natural Science Foundation of China(82430032);National Key Research and Development Program of China(2024YFC2510700);Shanghai Science and Technology Innovation Action Plan International Science and Technology Cooperation Program(23410713600);Natural Science Foundation of Shanghai(21ZR1436900);Cross-Disciplinary Research Fund of Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine(JYJC202116);Biomaterials and Regenerative Medicine Institute Cooperative Research Project, Shanghai Jiao Tong University School of Medicine(2022LHB02)

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Abstract:

Objective ·To investigate the regulatory effects and underlying mechanisms of mouse mandibular osteoblasts on B cell differentiation and development. Methods ·Single-cell suspensions from mouse mandibular bone were prepared using an optimized enzymatic digestion method and induced to differentiate into osteoblasts in vitro. Osteogenic potential was validated by real-time quantitative PCR (RT-qPCR), alkaline phosphatase (ALP) staining, and alizarin red S (ARS) staining. The spatial localization relationship between osteoblasts and B cells in mandibular tissues was examined via immunofluorescence staining. High-purity hematopoietic progenitor cells were isolated using fluorescence-activated cell sorting. A Transwell co-culture system was established to assess the regulatory effects of different osteoblast concentrations (5×104, 2.5×105, and 5×105 cells/well) on B cell differentiation (5×104 cells/well). Flow cytometry and RT-qPCR were employed to evaluate B cell viability and differentiation. Additionally, RT-qPCR was used to analyze the expression of osteoblast-secreted factors associated with B cell development during osteogenic differentiation. Results ·Mandibular osteoblasts exhibited robust osteogenic potential, as confirmed by ALP/ARS staining and high expression of osteogenic markers (Runx2, Osx, Ocn, and Alp) via RT-qPCR. Immunofluorescence revealed close spatial proximity between osteoblasts and B cells in mandibular tissues. In the co-culture system, osteoblasts promoted B cell differentiation in a concentration-dependent manner. RT-qPCR and immunofluorescence demonstrated that osteoblasts significantly upregulated key genes involved in B cell development (Ebf1, Rag1, Il7r, and Pax5; all P<0.001). Furthermore, osteoblast-derived factors (Il7, Baff, and Flt3l) were markedly elevated during osteogenic differentiation (all P<0.05). Conclusion ·Mandibular osteoblasts enhance B cell differentiation and development in a concentration-dependent manner, likely through secreting growth factors that upregulate critical B cell differentiation genes.

Key words: jaw osteoblasts, osteoimmunology, B lymphocytes, immunoregulation

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