›› 2010, Vol. 30 ›› Issue (2): 240-.

• Technique and method • Previous Articles     Next Articles

Synthesis of substrate for angiotensin-converting enzyme and its value in diagnosis of lung cancer

ZHOU Hai-yan1, YAO Li-yun2, LI Ying-yi3, ZHANG Jian-hua2   

  1. 1. Department of Clinical Laboratory, Shanghai Chest Hospital, Shanghai Jiaotong University, Shanghai 200030, China；2. Department of Chemistry, Basic Medical College, Shanghai Jiaotong University, Shanghai 200025, China；3. Office of Counselor, School of Medicine, Shanghai Jiaotong University, Shanghai 200025, China
  • Online:2010-02-25 Published:2010-02-25
  • Supported by:

    Shanghai Municipal Health Bureau Foundation, 2007085

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Abstract:

Objective To establish the synthetic method of the substrate for angiotensin-converting enzyme (ACE), and investigate the assay method of serum ACE. Methods The substrate was prepared by the use of N-hydroxysuccinimide ester in peptide synthesis, and serum ACE was determined by spectrophotometric assay using substrate. Results The substrate of furanacryloyl-phenylalanyl-glycyl-glycine with purity of 99.5% was obtained. The Km value for the substrate was 0.25 mmol/L, the normal reference interval for serum ACE activity was 46.7 U/L to 113.3 U/L, and the within-run and between-run coefficient of variation (CVs) ranged from 3.9 to 4.9%. The serum ACE activity in patients with squamous carcinoma and adenocarcinoma of lung were significantly decreased (P<0.05), while that in patients with lung small cell carcinoma was not significantly decreased (P>0.05). Conclusion The synthesized substrate could be reliably used as a marker for diagnosis of different lung cancers in clinics.

Key words: angiotensin converting enzyme, furanacryloyl-phenylalanyl-glycyl-glycine, lung cancer