›› 2011, Vol. 31 ›› Issue (1): 15-.doi: 10.3969/j.issn.1674-8115.2011.01.004

• Original article (Basic research) • Previous Articles     Next Articles

Biocompatibility of fluorescent nanoparticles NaYF4:Yb,Er as imaging media

YU Yong-jiang1, MA Xiao-rong1, YU Guo-peng1, GAO Tong-bin1, QI Juan1, CHEN Fang2   

  1. 1.Department of Urology, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200092, China；2.Department of Urology, Shanghai Children's Hospital, Shanghai Jiaotong University, Shanghai 200040, China
  • Online:2011-01-28 Published:2011-02-01
  • Supported by:

    Foundation from Xinhua Hospital, Shanghai Jiaotong University School of Medicine, 09QYJ03

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Abstract:

Objective To investigate the biocompatibility of upconversion fluorescent nanoparticles in vivo and in vitro, and verify its safety as imaging media. Methods Mouse bone mesenchymal stem cells (BMSC), mouse embryonic fibroblasts (NIH/3T3) and primary myoblasts (C2C12) were incubated with different concentrations of NaYF4:Yb,Er (0,10,50,100 and 200 μg/mL). Cell proliferation was determined by MTT assay, and the formation of myotube cells from C2C12 myoblasts was detected. DMEM with NaYF4:Yb,Er nanoparticles were injected into C57BL/6 mice, and liver function and renal function were examined. HE staining was performed for main body organs, and toxicity was detected. Results MTT assay revealed that the cytotoxicity of NaYF4:Yb,Er on NIH/3T3 and C2C12 was positively correlated with incubation dose and time （NIH/3T3: r=0. 974, P<0.05; C2C12: r=0. 996, P<0.05）, while the same result was not found for BMSC (r=-0.218, P>0.05).The formation of myotube cells from C2C12 myoblasts was not significantly affected by incubation with NaYF4:Yb,Er for 48 h. No obvious damage of liver and renal function and main body organs was observed after injection of DMEM with NaYF4:Yb,Er nanoparticles in mice. Conclusion As biological luminescent labels with strong intensity, NaYF4: Yb, Er has less toxicity both in vivo and in vitro to the requirement of imaging, and is an ideal biological imaging media.

Key words: upconversion fluorescent nanoparticles, NaYF4:Yb,Er, biological imaging media, biocompatibility