Journal of Shanghai Jiao Tong University (Medical Science) >

2025 , Vol. 45 >Issue 9: 1106 - 1115

DOI: https://doi.org/10.3969/j.issn.1674-8115.2025.09.003

Basic research

Effect of jaw osteoblasts on B cell development via cytokine secretion

  • WANG Xinyu ,
  • CHEN Qianye ,
  • SUN Jiping ,
  • LU Tingwei ,
  • HUANG Xiangru ,
  • SUN Siyuan ,
  • LIU Yuanqi ,
  • PAN Houwen ,
  • DAI Qinggang ,
  • SHEN Lei ,
  • JIANG Lingyong
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  • 1.Department of Oral and Maxillofacial Surgery, Shanghai Ninth People′s Hospital, Shanghai Jiao Tong University School of Medicine; College of Stomatology, Shanghai Jiao Tong University; National Center for Stomatology; National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology; Shanghai Research Institute of Stomatology, Shanghai 200011, China
    2.Department of Oral Maxillofacio-Head and Neck Oncology, Shanghai Ninth People′s Hospital, Shanghai Jiao Tong University School of Medicine; College of Stomatology, Shanghai Jiao Tong University; National Center for Stomatology; National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology; Shanghai Research Institute of Stomatology, Shanghai 200011, China
    3.Department of Immunology and Microbiology, Shanghai Jiao Tong University School of Basic Medical Sciences; Shanghai Institute of Immunology, Shanghai 200025, China
    4.Department of 2nd Dental Centre, Shanghai Ninth People′s Hospital, Shanghai Jiao Tong University School of Medicine; College of Stomatology, Shanghai Jiao Tong University; National Center for Stomatology; National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology; Shanghai Research Institute of Stomatology, Shanghai 200011, China
JANG Lingyong, E-mail: jianglingyong@sjtu.edu.cn.

Received date: 2025-02-19

  Accepted date: 2025-04-29

  Online published: 2025-09-30

Supported by

“Two-Hundred Talents” Program of Shanghai Jiao Tong University School of Medicine(20221809);National Natural Science Foundation of China(82430032);National Key Research and Development Program of China(2024YFC2510700);Shanghai Science and Technology Innovation Action Plan International Science and Technology Cooperation Program(23410713600);Natural Science Foundation of Shanghai(21ZR1436900);Cross-Disciplinary Research Fund of Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine(JYJC202116);Biomaterials and Regenerative Medicine Institute Cooperative Research Project, Shanghai Jiao Tong University School of Medicine(2022LHB02)

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Abstract

Objective ·To investigate the regulatory effects and underlying mechanisms of mouse mandibular osteoblasts on B cell differentiation and development. Methods ·Single-cell suspensions from mouse mandibular bone were prepared using an optimized enzymatic digestion method and induced to differentiate into osteoblasts in vitro. Osteogenic potential was validated by real-time quantitative PCR (RT-qPCR), alkaline phosphatase (ALP) staining, and alizarin red S (ARS) staining. The spatial localization relationship between osteoblasts and B cells in mandibular tissues was examined via immunofluorescence staining. High-purity hematopoietic progenitor cells were isolated using fluorescence-activated cell sorting. A Transwell co-culture system was established to assess the regulatory effects of different osteoblast concentrations (5×104, 2.5×105, and 5×105 cells/well) on B cell differentiation (5×104 cells/well). Flow cytometry and RT-qPCR were employed to evaluate B cell viability and differentiation. Additionally, RT-qPCR was used to analyze the expression of osteoblast-secreted factors associated with B cell development during osteogenic differentiation. Results ·Mandibular osteoblasts exhibited robust osteogenic potential, as confirmed by ALP/ARS staining and high expression of osteogenic markers (Runx2, Osx, Ocn, and Alp) via RT-qPCR. Immunofluorescence revealed close spatial proximity between osteoblasts and B cells in mandibular tissues. In the co-culture system, osteoblasts promoted B cell differentiation in a concentration-dependent manner. RT-qPCR and immunofluorescence demonstrated that osteoblasts significantly upregulated key genes involved in B cell development (Ebf1, Rag1, Il7r, and Pax5; all P<0.001). Furthermore, osteoblast-derived factors (Il7, Baff, and Flt3l) were markedly elevated during osteogenic differentiation (all P<0.05). Conclusion ·Mandibular osteoblasts enhance B cell differentiation and development in a concentration-dependent manner, likely through secreting growth factors that upregulate critical B cell differentiation genes.

Key words： jaw osteoblasts; osteoimmunology; B lymphocytes; immunoregulation

Cite this article

WANG Xinyu , CHEN Qianye , SUN Jiping , LU Tingwei , HUANG Xiangru , SUN Siyuan , LIU Yuanqi , PAN Houwen , DAI Qinggang , SHEN Lei , JIANG Lingyong . Effect of jaw osteoblasts on B cell development via cytokine secretion[J]. Journal of Shanghai Jiao Tong University (Medical Science), 2025 , 45(9) : 1106 -1115 . DOI: 10.3969/j.issn.1674-8115.2025.09.003

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