上海交通大学学报(医学版)

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木犀草素对急性免疫性肝损伤小鼠的保护作用

张 琦   

  1. 上海交通大学医学院附属第九人民医院检验科, 上海 200011
  • 出版日期:2014-03-28 发布日期:2014-04-02
  • 作者简介:张 琦(1970—), 女, 主管技师; 电子信箱: zhangqi2228@hotmail.com。
  • 基金资助:

    国家自然科学基金(81200299)

Protective effects of luteolin on immune-mediated liver injury in mice

ZHANG Qi   

  1. Clinical Laboratory, the Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China
  • Online:2014-03-28 Published:2014-04-02
  • Supported by:

    National Natural Science Foundation of China,81200299

摘要:

目的 探讨木犀草素对免疫介导的肝损伤的保护作用。方法 C57BL/6小鼠随机分为实验组和对照组(n=5),利用刀豆蛋白A(ConA)尾静脉注射诱导建立免疫性肝损伤小鼠模型。实验组小鼠建模前经腹腔注射木犀草素预处理。全自动生化分析仪检测血清丙氨酸转氨酶(ALT)和天冬氨酸转氨酶(AST)水平;苏木精-伊红染色(HE染色)光学显微镜观察小鼠肝组织学改变,TUNEL检测分析细胞凋亡;酶联免疫吸附试验(ELISA)检测血清肿瘤坏死因子-α (TNF-α)和γ干扰素 (IFN-γ)水平。结果 实验组小鼠血清ALT和AST水平显著低于对照组(P<0.001);与对照组比较,实验组小鼠肝脏炎症细胞浸润情况较轻,肝细胞发生凋亡较少;实验组小鼠血清TNF-α和IFN-γ水平显著低于对照组(P<0.05和P<0.01)。结论 木犀草素预处理能够降低ConA诱导的炎症介质的分泌,减轻小鼠免疫性肝损伤。

关键词: 木犀草素, 刀豆蛋白A, 免疫介导的肝损伤, 炎症介质

Abstract:

Objective To investigate the protective effects of luteolin on immune-mediated liver injury in mice. Methods The mouse model of immune-mediated liver injury was established by ConA injection to C57BL/6 mouse. Serum ALT and AST levels in mice were determined by an automatic biochemistry analyzer. HE stain and TUNEL stain were used to determine the extent of liver injury. Serum TNF-α and IFN-γ concentrations were measured by ELISA. Results Luteolin treatment significantly decreased the ALT and AST levels in ConA-induced hepatitis (CIH) mice (P<0.001). Luteolin treated mice also displayed less inflammatory infiltration in the liver and hepatocyte apoptosis. Meanwhile, luteolin significantly inhibited the release of inflammatory cytokines such as TNF-α and IFN-γ in ConA injected mice (P<0.05, P<0.01). Conclusion Luteolin can protect mice from immune-mediated liver injury by inhibiting the release of ConA-induced inflammation mediator.

Key words: luteolin, ConA, immune-mediated liver injury, inflammation mediator