上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（基础研究） • 上一篇    下一篇

姜黄素不能抑制无镁人工脑脊液诱发大鼠海马切片产生的癫样放电

常燕燕1，宫新伟2，龚海庆2，张溥明2，梁培基2，陆钦池1   

  1. 上海交通大学  1.医学院附属仁济医院神经内科， 上海 200127； 2.生物医学工程学院， 上海 200240
  • 出版日期:2014-09-28 发布日期:2014-09-26
  • 通讯作者: 陆钦池, 电子信箱: qinchilu@yahoo.com。
  • 作者简介:常燕燕（1986—）， 女， 硕士生; 电子信箱: chaonhm@sjtu.edu.cn。
  • 基金资助:

    上海交通大学“医工（理） 交叉研究基金”（YG2012ZD08）

Failure of Curcumin in inhibiting epileptiform discharge of hippocampal slices of rat induced by Mg2+-free artificial cerebrospinal fluid

CHANG Yan-yan1, GONG Xin-wei2, GONG Hai-qing2, ZHANG Pu-ming2, LIANG Pei-ji2, LU Qin-chi1   

  1. 1.Department of Neurology, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200127, China; 2.School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China
  • Online:2014-09-28 Published:2014-09-26
  • Supported by:

    Shanghai Jiao Tong University Fund for Interdisciplinary Research for Medical Applications， YG2012ZD08

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摘要：

目的 通过与抗癫药物丙戊酸（VPA）的作用比较，探讨姜黄素对癫样放电活动有无直接抑制作用。方法 利用多电极记录系统记录无镁人工脑脊液（ACSF）诱发大鼠海马切片产生的癫样放电。成功诱导出癫样放电后，加入100 μmol/L姜黄素或3 mmol/L丙戊酸，观察其对癫样放电的作用。结果 无镁ACSF持续灌流15 min左右，多电极阵列上可记录到海马各区域的癫样放电。100 μmol/L姜黄素对癫样放电的放电频率、持续时间、传播速度等均无明显影响（P>0.05）。3 mmol/L VPA能使癫样放电的放电频率降低（P<0.05），逆行方向的传播速度降低（P<0.05）。结论 100 μmol/L姜黄素对无镁ACSF诱发的大鼠海马切片的癫样放电无抑制作用。

关键词: 癫样放电, 多电极阵列, 姜黄素, 丙戊酸, 海马切片

Abstract:

Objective To investigate whether curcumin has direct inhibitory effect on the epileptiform discharge by comparing the effects of curcumin and antiepileptic drug of valproate. Methods The epileptiform discharge of hippocampal slices of the rat induced by Mg2+-free artificial cerebrospinal fluid (ACSF) was recorded by the multi-electrode array (MEA). After epileptiform discharge was successfully induced, curcumin of 100 μmol/L or valproate of 3 mmol/L was applied and their effects on the epileptiform discharge were observed. Results After Mg2+-free ACSF was continuous perfused for 15 min, the epileptiform discharge of all regions of hippocampal slices was recorded by the MEA. Curcumin of 100 μmol/L did not significantly affect the frequency, duration, and propagation speed of epileptiform discharge (P>0.05). Valproate of 3 mmol/L significantly decreased the frequency of epileptiform discharge (P<0.05) and the propagation speed at retrograde direction (P<0.05). Conclusion Curcumin of 100 μmol/L fails to inhibit the epileptiform discharge of hippocampal slices of the rat induced by Mg2+-free ACSF.

Key words: epileptiform discharge, micro-electrode array, curcumin, valproate, hippocampal slice