上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（基础研究） • 上一篇    下一篇

嵌入式超声肿瘤温热治疗仪联合聚乙二醇化脂质体阿霉素干预后的兔VX2肿瘤模型中药物浓度的研究

赵银珠1，熊屏1，陈亚珠2   

  1. 1.上海交通大学 医学院附属第九人民医院超声医学科， 上海 200011； 2.上海交通大学 生物医学工程学院， 上海 200030
  • 出版日期:2015-08-28 发布日期:2015-09-30
  • 作者简介:赵银珠(1988—)， 女， 硕士生; 电子信箱: zhaoyinzhu106@163.com。
  • 基金资助:

    国家自然科学基金(81272567)

Study on drug concentration of rabbit VX2 tumor model intervened by combination of embedded ultrasound tumor hyperthermia instrument and pegylated liposomal doxorubicin

ZHAO Yin-zhu1, XIONG Ping1, CHEN Ya-zhu2   

  1. 1.Department of Ultrasound, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China; 2.School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200030, China
  • Online:2015-08-28 Published:2015-09-30
  • Supported by:

    National Natural Science Foundation of China, 81272567

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摘要：

目的  观察嵌入式超声肿瘤温热治疗仪联合聚乙二醇化脂质体阿霉素(PLD)干预后的兔VX2肿瘤模型中药物浓度的变化。方法  12只后肢浅层肌肉荷瘤新西兰雌兔于肿瘤各径线均大于10 mm时，随机分为超声热疗+PLD组、PLD组和阿霉素组，每组4只。其中超声热疗+PLD组在PLD药物注射完毕，即刻利用嵌入式超声肿瘤温热治疗仪给予肿瘤部位超声热疗(42 ℃~43 ℃) 30 min。采用高效液相色谱法(HPLC)确定肿瘤组织中阿霉素的浓度。结果  在超声热疗+PLD组，肿瘤加热区域获得精确的温度控制，为（42.5±0.2）℃；肿瘤组织中阿霉素浓度分别为PLD组和阿霉素组的2.0倍和11.2倍，差异均有统计学意义(P<0.05)。PLD组肿瘤组织中阿霉素浓度为阿霉素组的5.5倍，差异有统计学意义(P<0.05)。结论  嵌入式超声肿瘤温热治疗仪联合PLD能有效地提高兔VX2肿瘤模型中的药物浓度。

关键词: 嵌入式超声肿瘤温热治疗仪, 聚乙二醇化脂质体阿霉素, VX2肿瘤模型

Abstract:

Objective  To observe changes of the drug concentration of rabbit VX2 tumor model intervened by the combination of embedded ultrasound tumor hyperthermia instrument and pegylated liposomal doxorubicin (PLD). Methods  Twelve Zelanian female rabbits with VX2 tumors in superficial thigh muscle were randomly divided into the hyperthermia+PLD group (n=4), PLD group (n=4), and doxorubicin group (n=4) when the diameter of tumor was larger than 10 mm. For hyperthermia+PLD group, embedded ultrasound tumor hyperthermia instrument was used to perform ultrasound hyperthermia (42 ℃-43℃) for 30 min immediately after the injection of PLD drug. High-pressure liquid chromatography (HPLC) was adopted to determine the concentration of doxorubicin in tumor tissues. Results  The temperature of heated zone of the hyperthermia+PLD group was controlled accurately (42.5 ℃±0.2 ℃). The concentration of doxorubicin in tumor tissues of the hyperthermia+PLD group was 2.0 and 11.2 times higher than those of PLD group and doxorubicin group, respectively, and differences were statistically significant (P<0.05). The concentration of doxorubicin in tumor tissues of the PLD group was 5.5 times higher than that of the doxorubicin group and the difference was statistically significant (P<0.05). Conclusion  The combination of embedded ultrasound tumor hyperthermia instrument and PLD can effectively increase the drug concentration of the rabbit VX2 tumor model.

Key words: embedded ultrasound tumor hyperthermia instrument, pegylated liposomal doxorubicin, VX2 tumor model