人巨细胞病毒核酸定量荧光PCR检测的尿液室内质控物制备

doi:10.3969/j.issn.1674-8115.2015.11.034

上海交通大学学报（医学版）

人巨细胞病毒核酸定量荧光PCR检测的尿液室内质控物制备

陈克平1,2,徐玉蓉1,李丽1,2

1.东南大学附属中大医院临床检验中心，南京 210009； 2.东南大学医学院，南京 210009

出版日期:2015-11-28 发布日期:2016-01-13
作者简介:陈克平(1977—)，男，主管技师，博士; 电子信箱: keping2001@hotmail.com。
基金资助:
国家自然科学基金（81200877）

Preparation of an urine internal quality control substance for fluorescence PCR detection of cytomegalovirus DNA

CHEN Ke-ping1,2, XU Yu-rong1, LI Li1,2

1.Department of Clinical Laboratory, Zhongda Hospital, Southeast University, Nanjing 210009； 2.School of Medicine, Southeast University, Nanjing 210009

Online:2015-11-28 Published:2016-01-13
Supported by:
National Natural Science Foundation of China, 81200877

摘要/Abstract

摘要：

目的制备人巨细胞病毒核酸定量荧光PCR检测的尿液室内质控物，并观察其稳定性。方法收集荧光PCR检测结果为阳性的尿液标本，经简单过滤处理，-80 ℃保存观察其稳定性；并比较-20 ℃与-80 ℃保存条件对质控物稳定性的影响。结果在120次检测结果中，前20次采用即刻法，室内质控结果均在控；20次后绘制Levey-Jennings质控图，根据Westgard多规则评价室内质控物，结果均在控；-20 ℃与-80 ℃保存条件对制备的室内质控物稳定性无影响。结论简单方法制备的尿液室内质控物具有较好的稳定性，可以用作人巨细胞病毒核酸定量荧光PCR检测的室内质控物。

关键词: 人巨细胞病毒, 荧光PCR, 室内质控物

Abstract:

Objective To prepare an urine internal quality control substance for fluorescence PCR detection of cytomegalovirus DNA and observe the stability. Methods Urine samples with positive CMV-DNA were collected, filtered, and preserved at -80℃ to observe its stability. The effect of preservation conditions of -80 ℃ and -20 ℃ on the stability of quality control substance was compared. Results Among 120 tests, first 20 tests adopted the instance method and the results were under control. Levey-Jennings quality chart was plotted after 20 tests and the results were all under control according to Westgard rules. The preservation conditions of -80 ℃ and -20 ℃ did not affect the stability of prepared internal quality control substance. Conclusion The urine internal quality control substance prepared by simple method is stable and can be used as internal quality control substance for fluorescence PCR detection of cytomegalovirus DNA.

Key words: human cytomegalovirus, fluorescence PCR, internal quality control

陈克平,徐玉蓉,李丽. 人巨细胞病毒核酸定量荧光PCR检测的尿液室内质控物制备[J]. 上海交通大学学报（医学版）, doi: 10.3969/j.issn.1674-8115.2015.11.034.

CHEN Ke-ping, XU Yu-rong, LI Li. Preparation of an urine internal quality control substance for fluorescence PCR detection of cytomegalovirus DNA[J]. , doi: 10.3969/j.issn.1674-8115.2015.11.034.

人巨细胞病毒核酸定量荧光PCR检测的尿液室内质控物制备

Preparation of an urine internal quality control substance for fluorescence PCR detection of cytomegalovirus DNA

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