上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（基础研究） • 上一篇    下一篇

新型牙髓病治疗材料硅酸盐水泥的生物相容性评估

周志强1,2，张红梅2,3，林居红1,2，刘朝霞1，2   

  1. 重庆医科大学附属口腔医院 1.儿童口腔科, 2.口腔疾病与生物医学重庆市重点实验室, 3.重庆市高校市级口腔生物医学工程重点实验室， 重庆 401147
  • 出版日期:2016-04-28 发布日期:2016-05-26
  • 通讯作者: 刘朝霞, 电子信箱: chendelunwen@sina.com。
  • 作者简介:周志强（1990—）， 男， 硕士生; 电子信箱: 996421128@qq.com。
  • 基金资助:

    重庆市基础科学与前沿技术研究专项（cstc2014jcyjA10010）

Assessment of the biocompatibility of Portland cement， a novel  treatment material for endodontic disease

ZHOU Zhi-qiang1,2, ZHANG Hong-mei2,3, LIN Ju-hong1,2, LIU Zhao-xia1,2   

  1. 1.Department of Pediatric Dentistry, 2.Chongqing Key Laboratory for Oral Diseases and Biomedical Sciences, 3.Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Affiliated Hospital of Stomatology, Chongqing Medical University, Chongqing 401147， China
  • Online:2016-04-28 Published:2016-05-26
  • Supported by:

    Chongqing Research Program of Basic Research and Frontier Technology，cstc2014jcyjA10010

PDF (PC)

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摘要：

目的 评估硅酸盐水泥(PC)的生物相容性。 方法 参考GB/ T16175标准，将PC、无机三氧化聚合物(MTA)、无菌硅胶管（阴性对照组）直接埋入SD大鼠背部皮下，分别在术后7、30、90 d观察材料与组织界面的局部炎症反应。按GB/T16886标准，通过急性溶血实验评估材料的血液相容性。采用单因素方差分析比较组间炎症细胞数，LSD-t检验进行组间两两比较。结果 植入实验术后7 d，PC组、MTA组、阴性对照组炎症反应程度均表现为Ⅱ级炎症反应，PC组、MTA组炎症细胞数差异无统计学意义,但阴性对照组炎细胞数明显少于两实验组。30 d时，两实验组和阴性对照组炎症反应程度均较7 d时低，炎症反应表现为Ⅰ级炎症反应，3组间炎症细胞数差异无统计学意义。90 d时，各组炎症细胞数极少，各组间差异无统计学意义。溶血实验表明PC、MTA溶血率分别为1.02%、1.09%，符合小于5%的标准。结论 初步认为PC是一种生物相容性较好的生物材料。

关键词: 硅酸盐水泥, 无机三氧化聚合物, 生物相容性, 牙髓病, 治疗材料

Abstract:

Objective To assess the biocompatibility of Portland cement (PC). Methods According to GB/T16175 standard, we embedded the PC, mineral trioxide aggregate (MTA), and sterile silicone tube (negative control group) in the back of SD rats subcutaneously. Local inflammation at the interface between materials and tissue was observed 7, 30, and 90 d after embedment. The acute hemolytic experiment was conducted to assess the blood compatibility of materials according to GB/T16886 standard. One-way ANOVA was used to analyze the difference of inflammatory cell numbers among groups and LSD-t was used as multiple comparison methods. Results The PC group, MTA group and negative control group all showed a class Ⅱ inflammation reaction 7 d after embedment. There was no significant difference in inflammatory cell number between the PC group and the MTA group, however，the inflammatory cell number in the negative control group was significantly less than those in two experiment groups. All three groups showed a class Ⅰ inflammation reaction at 30 d, which was alleviated as compared with that seen at 7 d, meanwhile the inflammatory cell numbers in three groups had no significant difference. The inflammatory cells in all groups were very few with no difference between groups at 90 d. The hemolytic experiment showed that hemolysis rates in the PC group and the MTA group were 1.02% and 1.09%, which conformed to the standard of less than 5%. Conclusion It can be preliminarily concluded that PC is an biological material with favorable biocompatibility.

Key words: Portland cement； mineral trioxide aggregate； biocompatible, endodontic disease, treatment material