上海交通大学学报（医学版）

›› 2012, Vol. 32 ›› Issue (10): 1292-.doi: 10.3969/j.issn.1674-8115.2012.10.003

• 论著（基础研究） • 上一篇    下一篇

5-羟色胺对大鼠膀胱传入神经活动的影响

孙 俏, 孙碧英, 罗 萍, 王莹萍, 董 莉, 戎伟芳   

  1. 上海交通大学 |基础医学院生理学教研室, 上海 200025
  • 出版日期:2012-10-28 发布日期:2012-11-05
  • 通讯作者: 戎伟芳, 电子信箱: weifangrong@hotmail.com。
  • 作者简介:孙 俏（1987—）, 女, 硕士生；电子信箱: sunqiao_999@163.com。
  • 基金资助:

    上海市科委自然基金资助(10ZR1417300)

Effect of 5-hydroxytryptamine on activity of bladder afferent nerves in rats

SUN Qiao, SUN Bi-ying, LUO Ping, WANG Ying-ping, DONG Li, RONG Wei-fang   

  1. Department of Physiology, Basic Medical College, Shanghai Jiaotong University, Shanghai 200025, China
  • Online:2012-10-28 Published:2012-11-05
  • Supported by:

    Shanghai Science and Technology Committee Foundation, 10ZR1417300

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摘要：

目的 观察5-羟色胺（5-HT）对膀胱传入神经自发放电活动和机械敏感性的影响。方法 采用离体大鼠膀胱—盆神经标本，观察膀胱腔内给予不同浓度5-HT后，盆神经自发放电频率及充胀膀胱引起的放电活动的变化。结果 膀胱未充胀时，盆神经仅有少量自发放电活动（<10 imp/s）；充胀时，盆神经放电频率随膀胱内压力递增而升高；膀胱腔内给予5-HT （1～30 μmol/L, 0.1 mL）后，盆神经自发放电频率呈剂量依赖性增加，对0～15 mmHg范围（低压区）充胀刺激的反应增强。结论 5-HT能直接激活膀胱低阈值传入神经，并增强其对机械刺激的反应。

关键词: 5-羟色胺受体, 膀胱传入神经, 机械敏感性, 膀胱—盆神经标本

Abstract:

Objective To investigate the effect of 5-hydroxytryptamine (5-HT) on the spontaneous and mechanosensitive activity of bladder afferent nerves. Methods The effects of intravesical instillation of different concentrations of 5-HT on the spontaneous and distension-induced pelvic afferent nerve activity were observed in the isolated rat bladder/pelvic nerve preparations. Results At rest, with the bladder empty, there was little background activity (<10 imp/s) in the pelvic nerve branch innervating the bladder. During ramp distension, the afferent nerve activity increased progressively as the intravesical pressure rose. Intraluminal instillation of 0.1 mL 5-HT (1 to 30 μmol/L) caused an increase of the ongoing afferent discharge and potentiated the low threshold (0-15 mmHg) mechanosensory responses in a concentration-dependent manner. Conclusion 5-HT directly activates low threshold bladder afferent fibers in the pelvic nerve, and can potentiate their mechanosensitivity.

Key words: 5-hydroxytryptamine receptor, bladder afferent nerve, mechanosensitivity, bladder/pelvic nerve preparations