上海交通大学学报（医学版）

›› 2013, Vol. 33 ›› Issue (4): 392-.doi: 10.3969/j.issn.1674-8115.2013.04.002

• 论著（基础研究） • 上一篇    下一篇

亚低温干预对创伤性颅脑损伤大鼠海马组织CIB1表达的影响

殷玉华1,2, 李 明2, 贾 锋2, 徐 蔚1   

  1. 1.昆明医学院附属第二医院神经外科, 昆明 650101； 2.上海交通大学 医学院附属仁济医院神经外科, 上海 200127
  • 出版日期:2013-04-28 发布日期:2013-05-03
  • 通讯作者: 徐 蔚, 电子信箱: Xuwei_301@yahoo.com。
  • 作者简介:殷玉华(1965—）, 男, 主任医师, 博士生； 电子信箱: yinyuhuacn@yahoo.com.cn。
  • 基金资助:

    国家自然科学基金（81000829）

Effect of hypothermia on expression CIB1 in hippocampus after traumatic brain injury in rats

YIN Yu-hua1,2, LI Ming2, JIA Feng2, XU Wei1   

  1. 1.Department of Neurosurgery, the Second Affiliated Hospital of Kunming Medical College, Kunming 650101, China； 2.Department of Neurosurgery, Renji Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200127, China
  • Online:2013-04-28 Published:2013-05-03
  • Supported by:

    National Natural Science Foundation of China, 81000829

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摘要：

目的 研究亚低温干预对创伤性颅脑损伤（TBI）大鼠脑组织海马CA1区钙离子结合相关蛋白1（CIB1）表达的影响。方法 54只SD大鼠随机分为亚低温组（n=24）、常温组（n=24）和假损伤组（n=6)。亚低温组和常温组大鼠经液压侧方打击制作TBI模型，亚低温组大鼠建模后给予亚低温干预（32 ℃，4 h）。于TBI后4、6、12、24 h时（假损伤组为伤后24 h）分批（n=6）处死动物，取损伤侧海马CA1区组织，RT-PCR和Western blotting检测CIB1 mRNA 和蛋白的表达。结果 常温组和亚低温组大鼠TBI后各时点损伤侧海马CA1区组织中CIB1 mRNA和蛋白的表达均显著高于假损伤组（P<0.05），分别在伤后4h 和6 h上调达最高水平；亚低温组CIB1 mRNA和蛋白表达的上调程度显著低于常温组（P<0.05）。结论 亚低温干预对TBI大鼠海马组织CIB1 mRNA和蛋白的表达上调具有抑制作用。

关键词: 创伤性颅脑损伤, 亚低温, 钙离子结合相关蛋白1

Abstract:

Objective To investigate the effect of hypothermia on the expression of calcium and integrin-binding protein 1 (CIB1) in CA1 region of hippocampus after traumatic brain injury (TBI) in rats. Methods Fifty-four SD rats were randomly assigned to hypothermia group (n=24), normothermia group (n=24) and sham injury group (n=6). TBI models were established in hypothermia group and normothermia group, and hypothermia intervention (32 ℃，4 h) was conducted in hypothermia group. Rats in hypothermia group and normothermia group were sacrificed 4 h, 6 h, 12 h and 24 h after TBI, and those in sham injury group were sacrificed 24 h after injury, with 6 rats at each time point. Tissues in CA1 region of the injury side were obtained, and RT-PCR and Western blotting were adopted to detect the expression of CIB1 mRNA and protein. Results The expression of CIB1 mRNA and protein in CA1 region of hippocampus of the injury side in hypothermia group and normothermia group was significantly higher than that in sham injury group at each time point after injury (P<0.05), and the expression reached the peak at the time points of 4 h and 6 h after TBI. The expression of CIB1 mRNA and protein in hypothermia group was significantly lower than that in normothermia group (P<0.05). Conclusion Hypothermia treatment can suppress the upregulation of expression of CIB1 mRNA and protein in hippocampus after TBI in rats.

Key words: traumatic brain injury, hypothermia, calcium and integrin-binding protein 1