上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（基础研究） • 上一篇    下一篇

EMMPRIN在小鼠下颌第一磨牙形态发生及硬组织形成中的功能性作用

黄 立1，谢 明2   

  1. 1.福建医科大学 附属第一医院口腔颌面外科， 福州 350005； 2.上海交通大学 医学院附属第九人民医院口腔修复科， 上海 200011
  • 出版日期:2014-01-28 发布日期:2014-01-29
  • 通讯作者: 谢 明, 电子信箱: mingxiex@126.com。
  • 作者简介:黄 立（1976—）， 男， 主治医师， 硕士; 电子信箱: huangli_1222@126.com。
  • 基金资助:

    上海市重点学科建设项目（S30206）；上海市重点学科（特色学科）建设项目（T0202）； 国家自然科学基金（30900848）；福建省卫生厅青年科研基金（2010-1-16）

Functional role of EMMPRIN in morphogenesis of tooth germ and formation of hard tissues in mouse mandibular first molars

HUANG Li1, XIE Ming2   

  1. 1.Department of Maxillofacial Surgery, the First Affiliated Hospital, Fujian Medical University, Fuzhou 350005； 2.Department of Prosthodontics, the Ninth People′s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China
  • Online:2014-01-28 Published:2014-01-29
  • Supported by:

    Construction of Shanghai Key Disciplines Project, S30206; Construction of Shanghai Key Disciplines-Characteristic Disciplines Project, T0202; National Natural Science Foundation of China, 30900848; Foundation for Young Scientists of Fujian Health Bureau, 2010-1-16

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摘要：

目的 研究小鼠下颌第一磨牙中后期牙胚发育过程中细胞外基质金属蛋白酶诱导因子（EMMPRIN）的表达情况及其对牙齿细胞外基质分泌和矿化的影响。方法 采用免疫组织化学方法检测EMMPRIN在牙胚发育各个阶段中的表达定位；应用体外牙胚器官培养法以及抗体阻断的方法观察EMMPRIN功能性缺失对于牙胚形态发生的影响；采用Real-Time PCR技术检测EMMPRIN相关基因的表达变化。结果 EMMPRIN蛋白特异性地表达于牙胚的帽状期、钟状期以及其后的分泌期；阻断体外培养牙胚中EMMPRIN的蛋白活性可导致牙釉质形成障碍，同时下调牙胚培养物中EMMPRIN相关基因MMP-9、MMP-13、MMP-20、ALPL、ameloblastin、amelogenin和enamelin mRNA的表达。结论 EMMPRIN的表达贯穿于牙胚发生发育的多个阶段；EMMPRIN可能通过调控金属基质蛋白酶和（或）相关釉质基质蛋白而在成釉细胞的分化及釉质基质的分泌、矿化过程中发挥关键作用。

关键词: 细胞外基质金属蛋白酶诱导因子, 釉质发生, 牙胚

Abstract:

Objective To explore expression of extracellular matrix metalloproteinase inducer （EMMPRIN）during mid- and late-stage of mouse low first molar development and its effect on secretion and mineralization of dental matrices. Methods Immunohistochemistry was used to detect EMMPRIN expression in the developing tooth germ at different stages. Tooth germ culture was performed in association with EMMPRIN inhibition assay to observe the consequence of EMMPRIN defects on the morphogenesis of tooth germ. Meanwhile, the mRNA expression of EMMPRIN-related genes was examined by Real-Time PCR. Results Specific expression of EMMPRIN was detected in the tooth germs at cap stage, bell stage, as well as secretory stage. EMMPRIN abrogation resulted in the disturbance of amelogenesis and the downregulation of MMP-9, MMP-13, MMP-20, ALPL, ameloblastin, amelogenin, and enamelin mRNA expression in cultured tooth germ explants. Conclusion EMMPRIN was expressed in the different stages of tooth germ development. EMMPRIN played a critical role in the differentiation of ameloblast as well as the secretion and mineralization of enamel matrix, possibly by regulating the mRNA expression of MMPs and/or related enamel matrix proteins.

Key words: extracellular matrix metalloproteinase inducer, amelogenesis, tooth germ