上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（基础研究） • 上一篇    下一篇

人MⅡ期卵母细胞极体及原核期形态分级对预测胚胎发育潜能的研究

叶雅萍1，袁 华2，翟丹梅2，江 莉2   

  1. 1．广西医科大学研究生学院， 南宁 530021； 2．广西医科大学第一附属医院生殖医学研究中心， 南宁 530021
  • 出版日期:2014-03-28 发布日期:2014-04-02
  • 通讯作者: 袁 华, 电子信箱: yuan-hua2008@163.com。
  • 作者简介:叶雅萍（1984—）， 女， 在读硕士研究生; 电子信箱: yeyaping127268@126.com。
  • 基金资助:

    广西壮族自治区卫生厅自筹经费科研课题（桂卫Z2008140）

Research on indicating human embryo developmental potential by morphology of oocyte at metaphase Ⅱ （MⅡ）of the first polar body and pronucleus

YE Ya-ping1, YUAN Hua2, ZHAI Dan-mei2, JIANG Li2   

  1. 1.Postgraduate College, Guangxi Medical University, Nanning 530021, China; 2.Reproductive Medicine Research Center, the First Affiliated Hospital, Guangxi Medical University, Nanning 530021, China
  • Online:2014-03-28 Published:2014-04-02
  • Supported by:

    Self-raised Foundation of Department of Public Health of Guangxi Zhuang Autonomous Region, Z2008140

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摘要：

目的 对人MⅡ期卵母细胞的第一极体和D1双原核期的原核、核仁及胞浆进行形态分级，以预测胚胎发育潜能。方法 以2009年6月—2012年6月在广西医科大学第一附属医院生殖医学研究中心接受卵胞浆内单精子显微注射（ICSI）助孕治疗的269对不育夫妇（共269个移植周期）为研究对象。对所有获得的MⅡ期卵母细胞第一极体进行形态观察和分级，同时将D1双原核期的受精卵进行形态分级，分别比较其受精率、卵裂率、优质胚胎率、胚胎种植率及临床妊娠率。结果 根据所移植的胚胎来自不同级别第一极体的MⅡ期卵和不同原核等级分为B组和C组，分别为102例和98例，其余为A组（69例）。移植来源于1～2级第一极体的MⅡ期卵母细胞且D1双原核期为Z1～Z2级的胚胎其受精率、卵裂率、种植率、优胚率及临床妊娠率均较高。结论 人MⅡ期卵母细胞第一极体和D1双原核期受精卵的等级越高，胚胎发育的潜能就越好。从而提高ICSI的胚胎种植率及临床妊娠率。

关键词: MⅡ期卵, 第一极体形态, 原核形态, 种植率, 临床妊娠率, 胚胎发育潜能

Abstract:

Objective To evaluate the development of human embryos by morphology discrepancies of oocyte at metaphase Ⅱ (MⅡ) of the first polar body and pronucleus. Methods The 269 infertile couples (269 transfer cycles) who treated with intracytoplasmic sperm injection (ICSI） from June 2009 to June 2012 in the First Affiliated Hospital of Guangxi Medical University were selected. Oocytes at MⅡ were classified by the morphology of first polar body and pronucleus zygotes were classified by the Scott scoring system. Fertilization rate, cleavage rate, and good quality embryo rate of each type of oocytes and zygotes were campared. Results According to different grades of first polar body and pronucleus zygote, embryos were divided into group A (69 cases), group B (102 cases), and group C (98 cases). The embryos of oocytes (1-2 grade) and pronucleus (Z1-Z2 grade) had higher fertilization rate, cleavage rate, good quality embryo rate, implantation rate, and pregnancy rate. Conclusion The higher the grade of oocyte at metaphase Ⅱ of first polar body and pronucleus, the better potential of human embryo development. So this can increase the implantation and pregnancy rate of intracytoplasmic sperm injection/embryo transplantation.

Key words: metaphase Ⅱ oocyte, morphology of first polar body, pronuclei morphology, implantation rate, pregnancy rate, embryo development potention