上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（基础研究） • 上一篇    下一篇

抑制Bag1表达对胃癌细胞生长和化学治疗敏感性的影响

马 飞1,3，郑磊贞1，顾建春1，李小平1，张明迪2，王雅杰3   

  1. 1.上海交通大学 医学院附属新华医院肿瘤科， 上海 200092； 2.上海交通大学 医学院附属新华医院普外科， 上海 200092； 3.第二军医大学附属长海医院肿瘤科, 上海 200433
  • 出版日期:2014-03-28 发布日期:2014-04-02
  • 作者简介:马 飞（1976—）， 女， 硕士生; 电子信箱: irismafei@126.com。
  • 基金资助:

    上海交通大学医学院科技基金项目（12XJ22004)

Effects of Bag1 expression inhihition on cell growth and chemotherapy sensitivity in gastric cancer

MA Fei1,3, ZHENG Lei-zhen1, GU Jian-chun1, LI Xiao-ping1, ZHANG Ming-di2, WANG Ya-jie3   

  1. 1.Department of Oncology, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China; 2.Department of General Surgery, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China; 3.Department of Oncology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China
  • Online:2014-03-28 Published:2014-04-02
  • Supported by:

    Science and Technology Foundation of Shanghai Jiao Tong University School of Medicine, 12XJ22004

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摘要：

目的 探讨Bag1表达水平对胃癌细胞生长和对5-氟尿嘧啶（5-FU）敏感性的影响。方法 采用Real-Time PCR和Western blotting 检测Bag1在5个不同的胃癌细胞系中的表达水平。选取高表达Bag1的细胞系，采用慢病毒载体下调Bag1基础表达水平，下调效率经Real-Time PCR和Western blotting检测验证；采用CCK-8法检测Bag1表达水平下调对细胞生长及对5-FU敏感性的影响。结果 Bag1 mRNA和蛋白表达水平在SGC7901和KATO-Ⅲ细胞中均显著升高。有效下调Bag1的表达水平后，SGC7901和KATO-Ⅲ细胞增殖受到抑制，而对5-FU的敏感性显著提高。结论 沉默Bag1基因可显著抑制胃癌细胞增殖，增强细胞对5-FU的敏感性。Bag1可能成为胃癌化学治疗的新靶点。

关键词: 胃癌, Bag1, 细胞生长, 化学治疗, 敏感性

Abstract:

Objective To investigate the effects of Bag1 expression level on the growth of gastric cancer cells and chemo-sensitivity to 5-fluorouracil (5-FU). Methods The expression levels of Bag1 in five different gastric cancer cell lines were determined by Real-Time PCR and Western blotting. Cells with highly expressed Bag1 were selected and the basic expression level of Bag1 was down-regulated by the lentiviral shRNA. The efficiency of Bag1 silencing was confirmed by Real-Time PCR and Western blotting. The effects of the Bag1 knockdown on cell proliferation and chemo-sensitivity to 5-FU were detected by CCK-8 method. Results The expression levels of mRNA and protein of Bag1 were significantly increased in SGC7901 and KATO-Ⅲ cells. Efficient knockdown of Bag1 resulted in suppressing the proliferation of SGC7901 and KATO-Ⅲ cells and significantly enhancing the chemo-sensitivity to 5-FU. Conclusion Knockdown of Bag1 can significantly suppress the proliferation of gastric tumor cells and enhance chemo-sensitivity to 5-FU. Bag1 could be a new therapeutic target for chemotherapy of GC.

Key words: gastric cancer, Bag1, cell growth, chmotherapy, sensitivity