上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（临床研究） • 上一篇    下一篇

宫颈鳞状细胞癌EGFR、c-jun和c-fos表达与临床特征及近期疗效的相关性

赵俊玲，马栋辉，古丽娜·库尔班，王若峥   

  1. 新疆医科大学附属肿瘤医院放疗中心， 乌鲁木齐 830011
  • 出版日期:2015-02-28 发布日期:2015-02-27
  • 作者简介:赵俊玲(1985—)， 女， 硕士生; 电子信箱: zhaojunling0903@163.com。
  • 基金资助:

    科技部国际合作项目(2012DFA31560)

Correlation of expressions of EGFR, c-jun, and c-fos of cervical squamous cell carcinomas and clinical features and short-term efficacy

ZHAO Jun-ling, MA Dong-hui, Gulina·Kuerban, WANG Ruo-zheng   

  1. Radiotherapy Center, Affiliated Tumor Hospital of Xinjiang Medical University, Urumqi 830011, China
  • Online:2015-02-28 Published:2015-02-27
  • Supported by:

    International Cooperation Project of the Ministry of Science and Technology of China，2012DFA31560

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摘要：

目的 探讨宫颈鳞状细胞癌（CSCC）表皮生长因子受体（EGFR）、原癌基因c-jun、c-fos表达与临床特征及近期疗效的相关性。方法 荧光原位杂交（FISH）技术及免疫组织化学法检测60例CSCC组织EGFR基因拷贝数及EGFR、c-jun、c-fos蛋白表达水平。结果 EGFR基因拷贝数：二倍体32.2%、三倍体25.4%、多倍体30.5%、基因扩增率11.9%；EGFR、c-jun和c-fos蛋白阳性表达率分别为70.0%、76.7%和68.3%，三者均与肿瘤分化程度、淋巴结转移有关（P均<0.01）；EGFR蛋白表达与临床分期有关（P<0.01）；Spearman等级相关分析显示：EGFR蛋白表达水平与基因拷贝数增加呈正相关（r=0.622，P=0.000），c-jun和c-fos蛋白表达与临床分期呈正相关（r=0.293，P=0.023和r=0.296，P=0.022）；EGFR与c-jun、c-fos蛋白表达水平呈正相关（r=0.422，P=0.001；r=0.509，P=0.000）；EGFR、c-jun和c-fos蛋白表达对客观有效率无明显影响（P>0.05）。结论 EGFR、c-jun和c-fos表达可能为CSCC高危进展、转移的参考指标；三者可能均不是影响CSCC近期疗效的独立因素；c-jun和c-fos在EGFR介导的信号转导通路中可能发挥正性调节作用。

关键词: 宫颈鳞状细胞癌, 表皮生长因子受体, c-jun, c-fos, 近期疗效

Abstract:

Objective To investigate the correlation of expressions of epidermal growth factor receptor (EGFR) and proto-oncogenes c-jun and c-fos of cervical squamous cell carcinomas (CSCC) and clinical features and short-term efficacy. Methods The fluorescence in situ hybridization (FISH) and immunohistochemistry method were adopted to detect the number of EGFR gene copies and protein expression levels of EGFR, c-jun, and c-fos of CSCC tissues of 60 cases. Results The numbers of EGFR gene copies were 32.2% for diploid, 25.4% for triploid, 30.5% for polyploid, and 11.9% for gene amplification. The positive protein expression rates of EGFR, c-jun, and c-fos were 70.0%, 76.7%, and 68.3% and correlated with the lymph node metastasis and tumor differentiation (P<0.01). The protein expression of EGFR correlated with the clinical stages (P<0.01). Spearman rank correlation analysis showed that the protein expression level of EGFR positively correlated with the increase of gene copies (r=0.622, P=0.000); protein expressions of c-jun and c-fos positively correlated with the clinical stages (r=0.293, P=0.023; r=0.296, P=0.022); the protein expression level of EGFR positively correlated with protein expressions levels of c-jun and c-fos (r=0.422, P=0.001; r=0.509, P=0.000); and protein expressions of EGFR, c-jun, and c-fos did not significantly affect the objective efficiency (P>0.05). Conclusion Expressions of EGFR, c-jun, and c-fos may be reference indexes of the development and metastasis of CSCC and may not be independent factors that affect the short-term efficacy of CSCC. The c-jun and c-fos may play a positive regulatory role in EGFR-mediated signal transduction pathway.

Key words: cervical squamous cell carcinoma, epidermal growth factor receptor, proto-oncogene c-jun, proto-oncogene c-fos, short-term efficacy