上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（临床研究） • 上一篇    下一篇

循环CD34+ 细胞数量与老年高血压的关系

黄黎莹，沈培晓，汪海娅   

  1. 上海交通大学 医学院附属仁济医院老年病科， 上海 200127
  • 出版日期:2016-03-28 发布日期:2017-06-02
  • 通讯作者: 汪海娅, 电子信箱: why7848@163.com。
  • 作者简介:黄黎莹（1981—）， 女， 住院医师， 硕士; 电子信箱: liying1769@163.com。

Correlation between circulating CD34+ level and elderly patients with hypertension  

HUANG Li-ying, SHEN Pei-xiao, WANG Hai-ya   

  1. Department of Geriatrics, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200127, China
  • Online:2016-03-28 Published:2017-06-02

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摘要：

目的 探讨循环CD34+ 细胞水平与老年高血压关系，分析Ang-1/Tie-2 在内皮祖细胞的表达及其调控意义。方法 选择老年高血压患者59例为高血压组，正常健康者59例为对照组。RT-PCR法检测Ang-1/Tie-2 mRNA表达，应用流式细胞仪测定外周血CD34+/PMNC值。分析外周血CD34+/PMNC值与各临床指标的相关性，以及Ang-1和Tie-2 mRNA水平与CD34+/PMNC值的相关性。结果 高血压组外周血CD34+/PMNC值显著低于对照组（P=0.000）。高血压组外周血CD34+/PMNC值与收缩压呈负相关（r=-0.622,P=0.009），与舒张压、总胆固醇、三酰甘油、高密度脂蛋白胆固醇、低密度脂蛋白胆固醇、空腹血糖、尿酸水平无相关性。高血压组内皮祖细胞Ang-1和Tie-2 mRNA水平显著低于对照组（P=0.000），与CD34+/PMNC值分别呈正相关（r=0.561,P=0.005；r=0.473,P=0.009）。结论 老年高血压患者的心血管病变可能与Ang-1和Tie-2水平的下调从而影响内皮祖细胞的修复功能有关。

关键词: 老年高血压, 循环内皮祖细胞, CD34+

Abstract:

Objective To explore the correlation between circulating CD34+ cell level and elderly patients with hypertension and analyze Ang-1/Tie-2 expression in endothelial progenitor cells and its regulatory meaning. Methods Fiftynine elderly patients with hypertension and 59 healthy people were enrolled and assigned to the hypertension group and the control group, respectively. The mRNA level of Ang-1/Tie-2  was measured by RTPCR. Flow cytometry was used to detect the CD34+/PMNC value in peripheral blood. The correlations between CD34+/PMNC value in peripheral blood and clinical indexes and between Ang-1 and Tie-2  mRNA levels and CD34+/PMNC value were analyzed. Results The CD34+/PMNC value in peripheral blood of the hypertension group was significantly lower than that of the control group (P=0.000). The CD34+/PMNC value in peripheral blood of the hypertension group was negatively correlated to systolic pressure (r=-0.622; P=0.009), while was not correlated to diastolic pressure, total cholesterol, triglyceride, high density lipoprotein cholesterol, low density lipoprotein cholesterol levels, fasting blood glucose and uric acid. The Ang-1 and Tie-2 mRNA levels in endothelial progenitor cells of the hypertension group were significantly lower than those in the control group (P=0.000) and were positively correlated to CD34+/PMNC value （r=0.561,P=0.005；r=0.473,P=0.009）. Conclusion Cardiovascular lesions in elderly patients with hypertension may be associated with the downregulation of Ang-1 and Tie-2 levels, which affects repair function of endothelial progenitor cells.

Key words: elderly patients with hypertension, circulating endothelial progenitor cells, CD34+