上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（基础研究） • 上一篇    下一篇

枸杞多糖及其配伍茶多酚缓解小鼠体力疲劳作用的研究

张雅莉，黄晓旭，黄珍茹，高润颖，蔡美琴   

  1. 上海交通大学 公共卫生学院， 上海 200025
  • 出版日期:2016-05-28 发布日期:2016-05-26
  • 通讯作者: 蔡美琴, 电子信箱: caimeiqin@sjtu.edu.cn。
  • 作者简介:张雅莉（1990—）， 女， 硕士生; 电子信箱: abeney@sina.com。

Study on the effects of Lycium barbarum polysaccharides (LBP) and LBP combined with tea polyphenols on relieving the physical fatigue in mice

ZHANG Ya-li, HUANG Xiao-xu, HUANG Zhen-ru, GAO Run-ying, CAI Mei-qin   

  1. School of Public Health, Shanghai Jiao Tong University, Shanghai 200025
  • Online:2016-05-28 Published:2016-05-26

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摘要：

目的 评价枸杞多糖（LBP）及LBP配伍茶多酚（TP）缓解小鼠体力疲劳作用的效果，并探讨其作用机制。方法 SPF级雄性昆明小鼠336只，共分为4批，每批随机分成7组：LBP低、中、高剂量(LBP-L、LBP-M、LBP-H)组，LBP配伍TP（以下简称配伍组）低、中、高剂量(C-L、C-M、C-H)组，设生理盐水(NS)为对照，每组12只。小鼠在每日饲喂基础饲料的基础上，以每10 g体质量0.2 mL溶液灌胃，15 d后进行负重游泳实验，并检测肝糖原、血清尿素氮和血乳酸等生化指标。结果 同NS组相比，LBP-M、LBP-H组小鼠负重游泳时间显著延长（P<0.05），LBP-L、LBP-M、LBP-H组肝糖原的含量显著增加（P<0.05），血清尿素氮和血乳酸水平显著降低（P<0.05）；C-L、C-M、C-H组小鼠负重游泳时间显著延长（P<0.05），肝糖原的含量显著提高（P<0.05），血清尿素氮和血乳酸水平显著降低（P<0.05）。同LBP组相比，配伍组负重游泳时间延长，肝糖原含量增加，血尿素氮和血乳酸水平降低；其中C-L组肝糖原水平显著高于LBP-L组（P=0.027），C-M组血乳酸水平显著低于LBP-M组（P=0.006）。结论 LBP及LBP配伍TP对小鼠体质量无影响，但具有缓解小鼠体力疲劳的作用；其作用机制可能与其增加了肝糖原储备量、减少蛋白质的分解代谢，进而减少血清尿素氮水平及增加了磷酸果糖激酶的活性，使血乳酸水平下降有关。

关键词: 枸杞多糖, 茶多酚, 配伍, 体力疲劳

Abstract:

Objective To evaluate the effects of Lycium barbarum polysaccharides (LBP) and LBP combined with tea polyphenols (TP) on relieving the physical fatigue in mice and discuss relevant mechanisms. Methods Three hundreds and thirty-six specific pathogen free (SPF) male KM mice were randomly assigned to four groups and each group was further divided into seven subgroups, i.e. three LBP groups receiving low, medium, and high doses of LBH (LBP-L, LBP-M, and LBP-H groups) and three combination groups receiving low, medium, and high doses of LBP combined with TP, and a normal saline (NS) group as the control group with 12 mice in each group. Mice were gavaged with LBP solution or LBP combined with TP solution at 0.2 mL/10 g body weight daily for 15 d. Then load swimming tests were conducted and biochemical indexes such as hepatic glycogen, serum urea nitrogen, and blood lactic acid were measured. Results LBP-M, LBP-H, C-L, C-M, and C-H groups had prolonged load swimming time (P<0.05) and LBP-L, LBP-M, LBP-H, C-L, C-M, and C-H groups had elevated hepatic glycogen level (P<0.05) and reduced serum urea nitrogen and blood lactic acid levels (P<0.05) as compared with the NS group. The combination groups had prolonged load swimming time, elevated hepatic glycogen level, and reduced serum urea nitrogen and blood lactic acid levels as compared with the LBP group. The hepatic glycogen level in C-L group was significantly higher than that in LBP-L group (P=0.027) and the blood lactic acid level in C-M group was significantly lower than that in LBP-M group (P=0.006). Conclusion LBP and LBP combined with TP can relieve the physical fatigue in mice without affecting the mouse body weight. The mechanism may be associated with increased hepatic glycogen storage, reduced catabolism for proteins, reduced serum urea nitrogen level, increased phosphofructokinase activity, and decreased blood lactic acid level.

Key words: Lycium barbarum polysaccharides, tea polyphenols, compatibility, physical fatigue