上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（基础研究） • 上一篇    下一篇

聚对二氧环己酮与碘海醇共混物生物相容性研究

刘惠东，刘洋，陈笋   

  1. 上海交通大学 医学院附属新华医院小儿心脏中心， 上海 200092
  • 出版日期:2016-05-28 发布日期:2016-05-26
  • 通讯作者: 陈笋, 电子信箱: drsunchen@vip.sina.com。
  • 作者简介:刘惠东（1990—）， 男， 硕士生; 电子信箱: lhd7607@163.com。
  • 基金资助:

    上海市科委产学研医合作项目（13DZ1942705，124119a3900）；上海高校工程研究中心（GCZX14011）

Study on the biocompatibility of the mixture of poly-p-dioxanone and iohexol

LIU Hui-dong, LIU Yang, CHEN Sun   

  1. Department of Pediatric Heart Center， Xinhua Hospital， Shanghai Jiao Tong University School of Medicine， Shanghai 200092， China
  • Online:2016-05-28 Published:2016-05-26
  • Supported by:

    Production-Study-Research-Medical Cooperation Projects， Science and Technology Commission of Shanghai Municipality， 13DZ1942705， 124119a3900； Shanghai Universities Engineering Research Center， GCZX14011

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摘要：

目的 研究聚对二氧环己酮（PPDO）与碘海醇通过物理共混方法制备显影材料的生物相容性。方法 平板硫化仪压制PPDO和PPDO-碘海醇混合物(MIX)成薄片。通过动态凝血时间试验、部分凝血活酶时间试验、血小板黏附试验和溶血试验评价材料的血液相容性。使用PPDO和MIX浸提液培养人脐静脉内皮细胞（HUVEC）和人主动脉平滑肌细胞（HA-VSMC），检测2种细胞的增殖情况。采用动物体内肌肉包埋法评价材料的组织相容性。结果 MIX与PPDO间血液相容性和组织相容性无明显差异。在细胞相容性评价中，HUVEC用MIX和PPDO浸提液培养,相对增长率分别为（80.26±8.87）%和（84.59±7.22）%（P=0.391）；而HA-VSMC的相对增长率分别为（94.89±9.64）%和（110.03±13.37）%（P=0.001）。结论 PPDO-碘海醇共混物具有良好的生物相容性。

关键词: 碘海醇, 聚对二氧环己酮, 生物相容性

Abstract:

Objective To investigate the biocompatibility of a developing material prepared by physically mixing poly-p-dioxanone (PPDO) and iohexol. Methods The PPDO and PPDO-iohexol mixture (MIX) were pressed into thin films by a plate vulcanization machine. The haemacompatibility was evaluated through dynamic clotting time test, partial thromboplastin time test, platelet adhesion test, and hemolysis test. The extracts of PPDO and MIX were used to culture human umbilical vein endothelial cells (HUVEC) and human aortic vascular smooth muscle cells (HA-VSMC). Cell proliferation of the two types of cells was detected. The histocompatibility was evaluated through in vivo muscle embed method. Results The differences in both haemacompatibility and histocompatibility between MIX and PPDO were not significant. In the cytocompatibility evaluation, the proliferation rates in HUVEC cultured with extracts of MIX and PPDO were (80.26±8.87)% and (84.59±7.22)% (P=0.391), respectively, while the proliferation rates in HA-VSMC were (94.89±9.64)% and (110.03±13.37)% (P=0.001), respectively.  Conclusion The mixture of PPDO and iohexol has excellent biocompatibility.

Key words: iohexol, poly-p-dioxanone, biocompatibility