上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（临床研究） • 上一篇    下一篇

6~7岁无龋与龋活跃儿童唾液糖蛋白水平的比较研究

王艳，李存荣，曾晓莉，徐玮，王勋，江一巍，张颖   

  1. 上海市口腔病防治院, 上海市口腔医院口腔预防处， 上海 200001
  • 出版日期:2016-06-28 发布日期:2016-07-25
  • 作者简介:王艳（1978—）， 女， 副主任医师， 博士; 电子信箱: serene2000@163.com。
  • 基金资助:

    上海市自然科学基金（13ZR1436200）；上海市卫生和计划生育委员会课题（20134028）

Comparative study on the level of salivary glycoproteins in 6-7 years old caries free and caries active children

WANG Yan, LI Cun-rong, ZENG Xiao-li, XU Wei, WANG Xun, JIANG Yi-wei,  ZHANG Ying   

  1. Department of Preventive Dentistry, Shanghai Stomatological Hospital, Shanghai 200001, China
  • Online:2016-06-28 Published:2016-07-25
  • Supported by:

    National Natural Science Foundation of Shanghai, 13ZR1436200; Foundation of Shanghai Manicial Commission of Health and Family Planning, 20134028

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摘要：

目的 研究6~7岁龋活跃儿童非刺激性全唾液中的糖蛋白水平，并与同年龄段无龋儿童进行比较。方法 选取50名6~7岁无龋[乳牙/恒牙龋失补牙数(dmft/DMFT)＝0）]儿童（无龋组）和50名6~7岁龋活跃(dmft/DMFT≥6)儿童（龋活跃组），分别收集非刺激性全唾液2 mL，取上清液经SDS-PAGE电泳后行高碘酸希尔法阿尔新蓝染色，记录每个样本的糖蛋白条带数量，采用Mann-Whitney U检验分析无龋组和龋活跃组糖蛋白条带数量的差异。结果 SDS-PAGE电泳的胶体经高碘酸希尔法阿尔新蓝染色后，样本中的糖蛋白条带被染成蓝色。Mann-Whitney U检验结果显示：无龋组和龋活跃组儿童非刺激性全唾液中糖蛋白条带数量秩均值分别为42.63和58.37，差异有统计学意义（P=0.004）。结论 6~7岁龋活跃儿童非刺激性全唾液中糖蛋白的水平显著高于同年龄的无龋儿童，提示唾液糖蛋白水平的升高可能与6~7岁儿童患龋率的升高有关。

关键词: 唾液, 糖蛋白, 龋病

Abstract:

Objective To explore the glycoproteins level in unstimulated whole saliva from 6-7 years old caries active children and compare the glycoproteins level between caries active children and caries free children. Methods Two milliliter unstimulated whole saliva was collected from each of 50 caries free (dmft/DMFT＝0) and 50 caries active (dmft/DMFT≥6) children aged 6-7 years. The supernatant of the saliva underwent SDS-PAGE protein electrophoresis. Then PAS alcian blue staining was used to stain glycoproteins. The number of stained glycoprotein bands in each sample was recorded. Mann-Whitney U test was used to analyze the difference in the numbers of stained glycoprotein bands between the caries free group and the cares active group. Results The glycoproteins bands in samples were stained blue after PAS alcian blue staining for the gel undergoing SDS-PAGE electrophoresis. Mann-Whitney U test revealed that rank means of the numbers of glycoprotein bands in unstimulated whole saliva from the caries free group and the cares active group were 42.63 and 58.37 respectively and the difference was statistically significant (P=0.004).  Conclusion The glycoproteins level in unstimulated whole saliva in 6-7 years old caries active children is significantly higher as compared with age-matched caries free children, suggesting that elevated glycoproteins level may be associated with increased caries incidence in children aged 6-7 years.

Key words: saliva, glycoprotein, caries