上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（基础研究） • 上一篇    下一篇

人毛囊隆突区上皮干细胞、黑素干细胞及自发荧光存在的研究

吴桂菊1， 侯强1， 顾军2， 刘继勇3   

  1. 1.上海市徐汇区大华医院皮肤科， 上海 200237； 2.上海长海医院皮肤科， 上海 200433； 3.上海长海医院药材科， 上海 200433
  • 出版日期:2016-10-28 发布日期:2016-11-29
  • 通讯作者: 侯强， 电子信箱： houqiang17@163.com。
  • 作者简介:吴桂菊（1984—）， 女， 主治医师， 硕士生； 电子信箱： drwuye@163.com。
  • 基金资助:

    上海市科学技术委员会生物医药领域产学研合作项目（13DZ1930906）

Study on the existence of epithelial stem cells, melanocyte stem cells, and autofluorescence in the bulge region of hair follicles

WU Gui-ju1， HOU Qiang1， GU Jun2， LIU Ji-yong3   

  1. 1.Department of Dermatology, Dahua Hospital, Shanghai 200237, China； 2.Department of Dermatology, Shanghai Changhai Hospital, Shanghai 200433, China； 3.Department of Pharmacy, Shanghai Changhai Hospital, Shanghai 200433, China
  • Online:2016-10-28 Published:2016-11-29
  • Supported by:

    Shanghai Science and Technology Committee Biomedical Field Production-Study-Research Cooperation Projects, 13DZ1930906

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摘要：

目的·探讨人毛囊隆突区是否存在上皮干细胞、黑素干细胞及自发荧光。方法·标本收集自整形外科健康志愿者后枕部和颞部全层头皮，分离完整毛囊。利用荧光显微镜观察毛囊自发荧光情况；采用间接免疫荧光法和免疫组织化学法检测毛囊角蛋白K15和K19的表达情况；采用RT-PCR检测毛球和隆突区上皮干细胞及黑素干细胞的标志物Dct、K15和K19，以及成熟细胞标志物Tyrase、Tyrp1、MC1R、MITF的mRNA水平。结果·人毛囊隆突区细胞可观察到自发荧光；免疫荧光和免疫组织化学检测结果显示隆突区外毛根鞘表达K15和K19；RT-PCR结果显示，隆突区Dct、K15的mRNA水平显著高于毛球，K19的mRNA水平与毛球相比无显著差异，而Tyrase、Tyrp1、MITF-M、MC1R的mRNA表达水平显著低于毛球。结论·人毛囊隆突区细胞可观察到自发荧光，且有上皮干细胞与黑素干细胞的存在。

关键词: 毛囊隆突区, 上皮干细胞, 黑素干细胞, 自发荧光区

Abstract:

Objective·To investigate whether there are epithelial stem cells, melanocyte stem cells, and autofluorescence in the bulge region of hair follicles. Methods·Samples were collected from the posterior occipital and temporal whole layer scalp of healthy volunteers with complete separation of hair follicles. Autofluorescence in hair follicles was observed using fluorescence microscope. Expressions of hair follicle keratins K15 and K19 were measured using indirect immunofluorescence and immunohistochemistry. RT-PCR was used to measure the mRNA expression levels of epithelial stem cell and melanocyte stem cell markers Dct, K15, and K19 and mature cell markers Tyrase, Tyrp1,MITF-M, and MC1R in hair bulbs and bulge region. Results·Autofluorescence was observed in the bulge region of hair follicles. Immunofluorescence and immunohistochemistry showed K15 and K19 were expressed in bulge region. RT-PCR showed that the mRNA expressions of Dct and K15 in bulge region were significantly higher than those in hair bulbs. The mRNA expression of K19 in bulge region was not significantly different from that in hair bulbs, while the mRNA expressions of Tyrase, Tyrp1, MITF-M, and MC1R in bulge region were significantly lower than those in hair bulbs. Conclusion·Autofluorescence can be observed and epithelial stem cells and melanocyte stem cells exist in the bulge region of hair follicles.

Key words: hair follicles bulge region, epithelial stem cell, melanocyte stem cell, autofluorescence