上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（基础研究） • 上一篇    下一篇

FLT3抑制剂AC220抑制眼新生血管的作用及机制

高誉硕，谢冰   

  1. 上海交通大学 医学院附属瑞金医院眼科， 上海 200025
  • 出版日期:2016-10-28 发布日期:2016-11-29
  • 通讯作者: 谢冰， 电子信箱： bingxie1@gmail.com。
  • 作者简介:高誉硕（1989—）， 女， 住院医师， 硕士； 电子信箱： 807746959@qq.com。

Inhibitory effect of FLT3 inhibitor AC220 on ocular neovascularization and its mechanisms

GAO Yu-shuo, XIE Bing   

  1. Department of Ophthalmology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China
  • Online:2016-10-28 Published:2016-11-29

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摘要：

目的·观察FMS-样酪氨酸激酶3（FLT3）与眼新生血管（NV）的关系，探讨FLT3抑制剂AC220对抗眼NV的作用机制。方法·建立小鼠氧诱导视网膜病变（OIR）模型和脉络膜新生血管（CNV）模型，采用免疫荧光法和酶联免疫吸附试验观察FLT3在OIR和CNV小鼠模型中的表达。在玻璃体腔内注射AC220，采用免疫荧光法观察其对眼NV的作用和对树突状细胞（DC）数目的影响。结果·FLT3在OIR和CNV模型小鼠视网膜的表达较对照组明显升高。玻璃体腔内注射AC220能有效减少OIR和CNV模型小鼠中眼NV的面积，并减少视网膜中DC的数目。结论·FLT3与眼NV密切相关。AC220具有抑制NV生成和调节DC数目的作用，有望成为临床抗NV治疗的辅助药物。

关键词: FMS-样酪氨酸激酶3, AC220, 眼新生血管, 脉络膜新生血管, 树突状细胞

Abstract:

Objective·To investigate association between FMS-like tyrosine kinase 3 (FLT3) and ocular neovascularization (NV) and explore the mechanisms of action of a novel FLT3 inhibitor AC220 in inhibiting ocular NV. Methods·A mouse model of oxygen induced retinopathy (OIR) and a mouse model of choroidal neovascularization (CNV) were constructed. FLT3 expressions in two models were detected by immumofluorescence method and ELISA. AC220 was intravitreally administrated and the effects of AC220 on ocular NV and the number of dendritic cell (DC) were investigated by immumofluorescence method. Results·The level of retinal FLT3 was significantly higher in the OIR and CNV groups than in the control group. Intravitreal administration of AC220 significantly reduced the ocular NV area in OIR and CNV groups and decreased the number of DC in retina. Conclusion·FLT3 is closely associated with ocular NV. AC220 can inhibit the development of NV, adjust the number of DC, and is a potential adjuvant medicine for anti-NV treatment.

Key words: FMS-like tyrosine kinase 3, AC220, retinal neovascularization, choroidal neovascularization, dendritic cell