上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（基础研究） • 上一篇    下一篇

分枝杆菌主要抗原成分对C57BL/6小鼠肝脏NKT细胞的免疫作用

刘毅 1, 2，王丹霓 1，雷航 1，史会影 1，孙妍 1，孙战强 3，张舒林 1   

  1. 1.上海交通大学基础医学院免疫学与微生物学系，上海 200025；2.上海健康医学院，上海 201318；3. 复旦大学遗传学研究所，上海 200433
  • 出版日期:2017-01-28 发布日期:2017-01-19
  • 通讯作者: 张舒林，电子信箱：shulinzhang@sjtu.edu.cn。
  • 作者简介:刘毅（1981—），男，助教，硕士生；电子信箱： billrommel@hotmail.com。
  • 基金资助:

    国家自然科学基金(81271794)

Immune effect of major antigen components of Mycobacterium on NKT cells in C57BL/6 mouse livers

LIU Yi1, 2, WANG Dan-ni1, LEI Hang1, SHI Hui-ying1, SUN Yan1, SUN Zhan-qiang3, ZHANG Shu-lin1   

  1. 1. Department of Microbiology and Immunology, Basic Medicine Faculty of Shanghai Jiao Tong University, Shanghai 200025,China; 2. Shanghai University of Medicine & Health Science, Shanghai 201318, China; 3. Institute of Genetics of Fudan University, Shanghai 200433, China
  • Online:2017-01-28 Published:2017-01-19
  • Supported by:

    National Nature Science Foundation of China，81271794

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摘要：

目的 ·评价分枝杆菌主要抗原成分对C57BL/6小鼠肝脏NKT细胞的免疫作用。方法 ·采用尾静脉注射法将牛分枝杆菌BCG活菌、全菌裂解物（WCL）、总脂抗原（TLIP）、培养滤液蛋白B（CFP-B）、脂聚糖（lipoglycan）分别免疫C57BL/6小鼠，免疫后第3、6、12日时分离小鼠肝脏细胞，用流式细胞术检测小鼠肝脏NKT细胞含量变化。以未经抗原免疫小鼠作为对照。结果 ·流式细胞术检测结果显示：对照组、lipoglycan组、CFP-B组、TLIP组、WCL组和BCG组小鼠肝脏内NKT细胞百分比分别为（0.040 0±0.020 62）%、（0.027 8±0.039 93）%、（0.035 6±0.047 46）%、（0.411 1±0.245 38）%、（0.118 9±0.054 19）%、（0.078 9±0.045 95）%。统计学分析结果显示：小鼠肝脏内NKT细胞百分比，TLIP组和WCL组显著高于对照组（P<0.01），且TLIP组高于WCL组；其余抗原组与对照组的差异无统计学意义（P>0.05）。结论 ·分枝杆菌主要抗原成分中，TLIP可显著增加C57/BL6小鼠肝脏内NKT细胞数量。TLIP具有潜在的抗结核免疫作用，值得进一步研究。

关键词: 分枝杆菌, NKT细胞, 脂抗原, 脂聚糖

Abstract:

 Objective · To evaluate the immune effect of major antigen components of Mycobacterium on NKT cells in C57BL/6 mouse livers. Methods · Mycobacterium bovis BCG cells, bacterial whole cell lysate (WCL), total lipid antigens (TLIP), culture filtrate protein B (CFP-B), and lipoglycan were used respectively to immunize C57BL/6 mice via tail vein injection. The liver cells were isolated 3, 6, and 12 days after immunization. Changes in NKT cell contents were measured with flow cytometry. Mice without antigen immunization were used as controls. Results · Flow cytometry showed that percentages of NKT cells in the control group, the lipoglycan group, the CFP-B group, the TLIP group, the WCL group, and the BCG group were (0.040 0±0.020 62)%, (0.027 8±0.039 93)%, (0.035 6±0.047 46)%, (0.411 1±0.245 38)%, (0.118 9±0.054 19)%, and (0.078 9±0.045 95)%, respectively. Statistical analysis showed that the content of NKT cells was significantly higher in the TLIP and WCL groups than in the control group (P<0.01) and was higher in the TLIP group than in the WCL group. The differences between other antigen groups and the control group were not statistically significant (P>0.05). Conclusion · Of major antigen components of Mycobacterium, TLIP can significantly increase the number of NKT cells in C57BL/6 mouse livers. TLIP is worthy of further investigation for its potential anti-tuberculosis immune function.

Key words: Mycobacterium, NKT cells, lipid antigen, lipoglycan