上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（临床研究） • 上一篇    下一篇

胃癌患者外周血miR-183水平的临床意义

孙颖1，马瑾1，胡梅洁2，郑雄1，李健1，顾玮1   

  1. 1. 上海交通大学医学院附属瑞金医院卢湾分院消化内科，上海 200020；2 .上海交通大学医学院附属瑞金医院消化内科，上海 200025
  • 出版日期:2017-01-28 发布日期:2017-01-19
  • 通讯作者: 顾玮，电子信箱：guwei1026@hotmail.com。
  • 作者简介:孙颖（1984—），女，主治医师，硕士；电子信箱：rjlwsy@163.com。
  • 基金资助:

    上海市黄浦区科研项目（HKW201303）

Level of miR-183 in peripheral blood of the patients with gastric cancer

SUN Ying1, MA Jin1, HU Mei-jie2, ZHENG Xiong1, LI Jian1, GU Wei1   

  1. 1. Department of Gastroenterology, Ruijin Hospital Luwan Branch, Shanghai Jiao Tong University School of Medicine, Shanghai 200020, China; 2. Department of Gastroenterology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China

  • Online:2017-01-28 Published:2017-01-19
  • Supported by:

    Scientific Research Project of Huangpu District，Shanghai, HKW201303

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摘要：

目的 ·研究胃癌患者外周血miR-183水平在胃癌的诊断及预后判断中的价值。方法 ·实时定量PCR(qRT-PCR)法检测76例胃癌患者及同期26例正常对照者外周血清miR-183的水平，分析miR-183水平与胃癌患者临床病理特征及预后的关系。结果 ·胃癌患者血清中miR-183水平明显高于正常对照者(4.798±0.267 vs 0.904±0.067，P=0.000)。miR-183水平与患者年龄、性别、肿瘤分化程度无明显相关性(P>0.05)；与肿瘤浸润深度、有无淋巴结转移或远处转移及TNM分期有关(P<0.05)。受试者工作特征曲线分析结果显示，miR-183在预测胃癌发生时，ROC曲线下面积（AUC）为0.978（95% CI为0.955～1.001），敏感度为93.42%，特异度为92.31%；在预测胃癌转移(淋巴结转移或远处转移)时，其AUC值为0.643(95% CI为0.507～0.780)，敏感度为67.31%，特异度为58.33%。结论 · miR-183在血清中的水平上调与胃癌发生、发展有关，胃癌患者血清中的表达上调与胃癌的临床病理特征及患者预后密切相关，miR-183可能成为胃癌诊断及预后判断的分子标志。

关键词: 微小RNA, miR-183, 胃癌, TNM分期

Abstract:

Objective · To evaluate level of miR-183 in peripheral blood of the patients with gastric cancer for diagnosis and prognosis. Methods · Quantitative real-time PCR was used to detect the miR-183 levels in sera of 76 cases of gastric cancer patients and 26 cases of normal control at the same term. The correlations of miR-183 levels with clinicopathological features and clinical prognosis were analyzed. Results · The significantly higher levels of miR-183 were observed in the sera of gastric cancer patients (4.798±0.267 vs 0.904±0.067, P=0.000) as compared with those in the corresponding control group. Up-regulation of miR-183 was associated with invasion depth, positive lymph node or distant metastasis, and advanced clinical stage in gastric cancer patients (P<0.05), but not associated with age, sex, and degree of tumor differentiation (P>0.05). ROC curve analysis showed that the AUC value of miR-183 in the prediction of gastric cancer was 0.978 (95% CI 0.955-1.001) with sensitivity of 93.42% and specificity of 92.31%. In the prediction of metastasis (lymph node metastasis or distant metastasis), the AUC value was 0.643 (95% CI 0.507-0.780) with sensitivity of 67.31% and specificity of 58.33%. Conclusion · The high level of miR-183 in serum is associated with gastric cancer development, its clinicopathological features and patient’s clinical prognosis. It may be used as a potential diagnostic bio-marker and a prognostic predictor in the patients with gastric cancer.

Key words: microRNA, miR-183, gastric cancer, TNM stage