上海交通大学学报（医学版）

上海交通大学学报（医学版） ›› 2019, Vol. 39 ›› Issue (2): 131-.doi: 10.3969/j.issn.1674-8115.2019.02.005

• 论著·基础研究 • 上一篇    下一篇

温敏性羟丁基几丁糖水凝胶预防新西兰大白兔宫腔粘连实验研究

吴若愚，曹忆梦，吴氢凯，邱雨，黄程胜，薛卓维   

  1. 宁夏医科大学公共卫生与管理学院，银川 750004
  • 出版日期:2019-02-28 发布日期:2019-03-19
  • 通讯作者: 吴氢凯，电子信箱：angelh2@163.com。
  • 作者简介:吴若愚 (1993—)，男，硕士生；电子信箱： roywxs@163.com。
  • 基金资助:
    国家自然科学基金（81550008，81771523）；上海市科学技术委员会产学研医合作项目（ 12DZ1940406）

Preventive effect of thermosensitive hydroxybutyl chitosan hydrogel in intrauterine adhesion in New Zealand white rabbits

WU Ruo-yu, CAO Yi-meng, WU Qing-kai, QIU Yu, HUANG Cheng-sheng, XUE Zhuo-wei   

  1. Department of Obstetrics and Gynecology, Shanghai Sixth Peoples Hospital, Shanghai Jiao Tong University, Shanghai 200233, China
  • Online:2019-02-28 Published:2019-03-19
  • Supported by:
    National Natural Science Foundation of China, 81550008, 81771523; Industry-University-Medical Research Cooperation Project of Shanghai Municipal Science and Technology Commission, 12DZ1940406

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摘要： 目的 ·观察温敏性羟丁基几丁糖（ hydroxybutyl chitosan，HBC）水凝胶预防新西兰大白兔宫腔粘连的效果。方法 ·将 18只雌性新西兰大白兔随机分为 3组，即正常对照组、模型组、 HBC组，每组 6只。正常对照组行假手术；模型组、 HBC组采用机械和感染双重损伤法构建宫腔粘连动物模型； HBC组建模后立即向宫腔内注射 2 mL 1.5%的温敏性 HBC水凝胶。每组分别于术后 1、2、4周各取 2只新西兰大白兔，收集双侧子宫组织，苏木精 -伊红染色观察子宫内膜形态及腺体数量； Masson染色检测子宫内膜纤维化程度。结果 ·与正常对照组相比，模型组与 HBC组术后 1周子宫内膜柱状上皮逐渐消失，纤维化面积比明显增加，腺体数量减少。 2周后，模型组低倍镜下可见宫腔内粘连形成，纤维化面积比进一步增加，腺体数量进一步减少；而 HBC组宫腔内未见水凝胶残余，纤维化面积比下降，腺体数量增加。 4周后， HBC组子宫内膜柱状上皮恢复，纤维面积比与腺体数量均恢复至正常水平。结论 ·温敏性 HBC水凝胶可以有效预防新西兰大白兔宫腔粘连形成。

关键词: 温敏性羟丁基几丁糖水凝胶, 宫腔粘连, 新西兰大白兔

Abstract:

Objective · To observe the effect of thermosensitive hydroxybutyl chitosan (HBC) hydrogel in the prevention of intrauterine adhesion in New Zealand white rabbits. Methods · Eighteen female New Zealand rabbits were randomly divided into 3 groups, i.e., normal control group, model group and HBC group. Normal control group underwent sham operation. The models of intrauterine adhesion were constructedboth mechanical damage and infection in model group and HBC group. In HBC group, 2 mL 1.5% thermosensitive HBC hydrogel was injected into the uterine cavity immediately after injury. Two rabbits were killed in each group 1 week, 2 weeks and 4 weeks after operation, respectively. The bilateral uterine tissues were collected. The endometrial morphology and quantity of glands were observedhematoxylin-eosin staining. The area of fibrosis in endometrium was measuredMasson staining. Results · One week after operation, compared with normal control group, the columnar epithelial cells of endometrium gradually disappeared and the ratio of endometrial fibrosis area increased significantly in the other two groups. The number of glands also decreased. After 2 weeks, intrauterine adhesion was observed in model group, and the ratio of endometrial fibrosis area continued increasing, and the number of glands decreased further. However, in HBC group, there was no residual hydrogel in the uterine cavity, and the ratio of endometrial fibrosis area decreased and the number of glands increased. After 4 weeks, there was a recovery of columnar epithelium cells, the ratio of endometrial fibrosis area, and the number of glands in HBC group, which returned to the normal level. Conclusion · Thermosensitive HBC hydrogel can effectively prevent intrauterine adhesion in New Zealand white rabbits.

Key words: thermosensitive hydroxybutyl chitosan hydrogel, intrauterine adhesion, New Zealand white rabbit

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