上海交通大学学报（医学版）

上海交通大学学报（医学版） ›› 2019, Vol. 39 ›› Issue (9): 933-.doi: 10.3969/j.issn.1674-8115.2019.09.001

• 论著·基础研究 • 上一篇    下一篇

奥氮平诱导体质量增加大鼠的肝脏代谢组学研究

汪也微，方新宇，陈艳，王丹丹，余玲芳，张晨   

  1. 上海交通大学医学院附属精神卫生中心生化研究室，上海 200030
  • 出版日期:2019-09-28 发布日期:2019-11-02
  • 通讯作者: 张晨，电子信箱：zhangchen645@gmail.com。
  • 作者简介:汪也微（1993—），女，硕士生；电子信箱： yuhanwang801@163.com。
  • 基金资助:
    国家自然科学基金（81000581，81471358，81771450）；国家重点研发计划（2018YFC1314302）；国家临床重点专科—上海市精神卫生中心（卫计委医政司 2011-873）；上海市科学技术发展基金（12140904200）；上海市教育委员会高峰高原学科建设计划（20152530）

Liver metabonomics analysis of olanzapine-induced weight gaining in rats

WANG Ye-wei, FANG Xin-yu, CHEN Yan, WANG Dan-dan, YU Ling-fang, ZHANG Chen   

  1. Biochemical Laboratory, Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine, Shanghai 200030, China
  • Online:2019-09-28 Published:2019-11-02
  • Supported by:
    National Natural Science Foundation of China, 81000581, 81471358, 81771450; National Key Research and Development Program, 2018YFC1314302; National Key Clinical Discipline at Shanghai Mental Health Center, OMA-MH 2011-873; Shanghai Science and Technology Development Fund, 12140904200; Shanghai Municipal Education Commission—Gaofeng Clinical Medicine Support, 20152530)。

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摘要： 目的 ·探究短期奥氮平给药后所引起的 SD大鼠体内代谢产物或代谢通路的变化。方法 ·采用 5周龄雌性 SD大鼠 20只，随机分为 2组，分别为空白对照组和奥氮平组。给药期间，监测 2组大鼠的体质量和摄食量。给药结束后，采集肝组织，并运用液相色谱 -质谱联用技术对肝组织中的代谢产物进行检测，后续转换原始数据，导入 MetaboAnalysis平台进行分析。结果 ·短期奥氮平给药会引起体质量、摄食量的显著增加以及糖耐量受损。代谢组学结果提示奥氮平组大鼠肝组织中初级胆汁酸和 9-氧代十八碳二烯酸（9-oxo-octadecadienoic acid, 9-OxoODE）水平显著高于空白对照组。结论 ·短期奥氮平给药会导致 SD大鼠体内初级胆汁酸生物合成过程的显著增强。

关键词: 奥氮平, 代谢组学, 肝组织, 液相色谱 -质谱联用

Abstract:

Objective · To observe the changes in metabolites and metabolic pathways in SD rats after olanzapine treatment. Methods · Twenty female SD rats were randomly assigned to two groups, control group and olanzapine treatment group, respectively. During the treatment period, body weight and food intake of two groups were monitored. At the end of treatment, the liver tissues were collected and LC/MS technique was applied to detect metabolites in liver tissue. Subsequently, the raw data were converted for further analysis on the MetaboAnalysis platform. Results · Short-term olanzapine administration resulted in significant increases in body mass, food intake, and impaired glucose tolerance. Metabonomics results indicated rats olanzapine treatment group had a significantly higher level of primary bile acid and 9-OxoODE (9-oxo-octadecadienoic acid) in liver tissue than those control group. Conclusion · Primary bile acid biosynthesis process is significantly enhanced after short-time olanzapine treatment.

Key words: olanzapine, metabonomics, liver tissue, liquid chromatography-mass spectrometry

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