上海交通大学学报（医学版）

›› 2009, Vol. 29 ›› Issue (10): 1196-.

• 论著（基础研究） • 上一篇    下一篇

Cop 1对实验性高眼压性青光眼视神经的保护作用

孙静芬, 王 玲   

  1. 上海交通大学 医学院瑞金医院眼科, 上海 200025
  • 出版日期:2009-10-25 发布日期:2009-10-26
  • 通讯作者: 王 玲, 电子信箱: lwang@rjh.com.cn。
  • 作者简介:孙静芬（1967—）, 女, 副主任医师, 博士；电子信箱: sunjingfen@sohu.com。

Neuroprotection of Copolymer 1 in glaucoma rats with elevated intraocular pressure

SUN Jing-fen, WANG Ling   

  1. Department of Ophthalmology, Ruijin Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200025, China
  • Online:2009-10-25 Published:2009-10-26

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摘要：

目的 观察Copolymer 1（Cop 1）对大鼠高眼压模型视神经的保护作用，为Cop 1在抗青光眼视神经萎缩的临床应用提供实验室依据。方法 将30只成年Wistar大鼠用Shareef-Sharma法制作双眼高眼压模型，在眼压升高的第3周，在Cop 1模型组（n=15）大鼠的后脚皮内注射100 μg Cop 1和等体积的Freund 完全佐剂（CFA）混合乳化液；在模型对照组（n=15）大鼠后脚皮内注射等体积的PBS和CFA混合乳化液，6 d后用Fluorogold进行视网膜神经节细胞（RGC）逆行性染色，1 d后取出视网膜，比较两组RGC密度的差异。另取6只正常大鼠（未做高眼压模型）作空白对照组。结果 Cop 1模型组视神经和视网膜切片的HE染色显示视神经大量淋巴细胞聚集，Cop 1模型组和模型对照组RGC的损失率分别为10.24%±3.75%和29.00%±6.92%；Cop 1模型组的RCG密度显著高于模型对照组（P＜0.05），但与空白对照组差异无统计学意义（P>0.05）。结论 Cop 1皮内注射能减少高眼压对RGC的损害。

关键词: Copolymer 1, 高眼压, 视网膜节细胞, 视神经保护

Abstract:

Objective To observe the neuroprotective effect of Copolymer 1(Cop 1) in rats with elevated intraocular pressure (IOP), and explore the clinical application of Cop 1 in the prevention of optic nerve atrophy induced by glaucoma. Methods Thirty adult Wistar rats were rendered for elevation of IOP in both eyes by Shareef-Sharma method. At the third week of IOP elevation, a mixture (1∶1) of Cop 1 (100 μg) emulsified in complete Freund's adjuvant (CFA) was injected intracutaneously at two hind footpads in 15 rats (Cop 1 model group), and the other 15 rats were given the same dose of PBS emulsified in CFA (model control group). Retinal ganglial cell (RGC) retrograde labeling was performed with Fluorogold 6 d later, and retina was taken after 24 h to compared the density of RGC between two groups. Another normal rats were served as blank controls. Results It was found that lymphocytes were accumulated on the histological slices of retina and optic nerve in Cop 1 model group. The rates of RGC loss of Cop 1 model group and model control group were 10.24%±3.75% and 29.00%±6.92%, respectively. The RGC density in Cop 1 model group was significantly lower than that in model control group (P＜0.05), while there was no significant difference in that between Cop 1 model group and blank control group (P>0.05). Conclusion Intracutaneous injection of Cop 1 can reduce the damage of RGC induced by elevated IOP.

Key words: Copolymer 1, elevated intraocular pressure, retinal ganglion cell, neuroprotection

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