上海交通大学学报（医学版）

›› 2010, Vol. 30 ›› Issue (1): 63-.

• 论著（基础研究） • 上一篇    下一篇

硝苯地平对药物性牙龈增生患者牙龈成纤维细胞增殖的影响

倪 靖, 束 蓉   

  1. 上海交通大学 医学院第九人民医院牙周科上海市口腔医学重点实验室, 上海 200011
  • 出版日期:2010-01-26 发布日期:2010-01-26
  • 通讯作者: 束 蓉, 电子信箱: shurong123@hotmail.com。
  • 作者简介:倪 靖（1983—）, 女, 硕士；电子信箱: natalie1229_9@126.com。
  • 基金资助:

    国家“十一五”科技支撑项目（2007BAI18B02）和上海市科委基金（08DZ2271100）

Effects of nifedipine on proliferation of gingival fibroblasts in drug-induced gingival hyperplasia

NI Jing, SHU Rong   

  1. Department of Periodontology, Shanghai Key Laboratory of Stomatology, Ninth People's Hospital, School of Medicine, Shanghai 200011, China
  • Online:2010-01-26 Published:2010-01-26
  • Supported by:

    National Science and Technology Pillar Program during the Eleventh Five-Year Plan Period, 2007BAI18B02；Foundation of Science and Technology Commission of Shanghai, 08DZ2271100

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摘要：

目的 研究硝苯地平致药物性牙龈增生患者牙龈成纤维细胞（NIFr-HGF）细胞周期增殖指数（PrI）的变化。方法 以服用硝苯地平发生牙龈增生患者的牙龈组织为实验对象。龈切术获得组织样本，组织块培养法分离培养NIFr-HGF，在细胞培养液中分别添加10 ng/mL和1 000 ng/mL硝苯地平进行干预（低、高浓度药物干预组），分别于干预后18 h和30 h时间点收集细胞，流式细胞仪检测细胞周期并计算PrI。以不添加硝苯地平的NIFr-HGF作为空白对照。结果 在药物作用时间相同条件下，两药物干预组NIFr-HGF细胞周期PrI明显高于空白对照组（P<0.05）；低浓度与高浓度药物干预组间NIFr-HGF细胞周期PrI比较差异无统计学意义（P>0.05）。在低浓度和高浓度药物干预组，干预30 h细胞周期的PrI明显高于干预18 h，分别为（57.54±0.019）% vs （21.15±0.011）%和（59.36±0.031）% vs （19.01± 0.012）% （P<0.05）。结论 对于硝苯地平致药物性牙龈增生患者，NIFr-HGF细胞周期PrI随硝苯地平作用时间延长而增大，而与药物浓度无明显关系。

关键词: 药物性牙龈增生, 硝苯地平, 牙龈成纤维细胞, 增殖指数

Abstract:

Objective To investigate the changes in proliferation index (PrI) of gingival fibroblasts in nifedipine-induced gingival hyperplasia (NIFr-HGF). Methods Gingival fibroblasts were derived from a patient with nifedipine-induced gingival hyperplasia. Cells were induced by 10 ng/mL and 1 000 ng/mL nifedipine (low- and high-concentration drug intervention groups), respectively. Cells were harvested 18 h and 30 h after intervention, cell cycles were detected by flow cytometry, and PrIs were calculated. NIFr-HGF without nifedipine induction were served as blank control. Results After induction for the same time, PrIs of NIFr-HGF cell cycle of low- and high-concentration drug intervention groups were significantly higher than those of blank control group (P<0.05), while there was no significant difference between low and high-concentration drug intervention groups (P>0.05). In low and high-concentration drug intervention groups, PrIs of NIFr-HGF cell cycle after intervention for 30 h were significantly higher than those after intervention for 18 h [(57.54±0.019)% vs (21.15±0.011)%, and (59.36±0.031)% vs (19.01±0.012)%, respectively] (P<0.05). Conclusion For patients with nifedipine-induced gingival hyperplasia, PrI of NIFr-HGF cell cycle increases with time of nifedipine intervention, while is not significantly related to drug concentration.

Key words: drug-induced gingival hyperplasia, nifedipine, gingival fibroblast, proliferation index