上海交通大学学报（医学版）

›› 2011, Vol. 31 ›› Issue (6): 764-.doi: 10.3969/j.issn.1674-8115.2011.06.018

• 论著（基础研究） • 上一篇    下一篇

选择性阻断cGMP依赖性蛋白激酶对内毒素孵育的大鼠血管环收缩功能的影响

郭 君1,2, 王学敏2, 支建明3, 王海燕2, 刘文涛1, 薛 瑛2   

  1. 1.苏州大学医学部, 苏州 215006； 2.上海交通大学附属第六人民医院麻醉科, 上海 200233； 3.上海交通大学 基础医学院生理学教研室, 上海 200025
  • 出版日期:2011-06-28 发布日期:2011-06-27
  • 通讯作者: 王学敏, 电子信箱: wangxm@sjtu.edu.cn。
  • 作者简介:郭 君 (1983—), 女, 硕士生；电子信箱: guojunfay@163.com。

Effects of selective blockade of cGMP-dependent protein kinase on contractility of vascular rings incubated with lipopolysaccharide in rats

GUO Jun1,2, WANG Xue-min2, ZHI Jian-ming3, WANG Hai-yan2, LIU Wen-tao1, XUE Ying2   

  1. 1.Medical College of Soochow University, Suzhou 215006, China；2.Department of Anesthesiology, the Sixth People's Hospital, Shanghai Jiaotong University, Shanghai 200233, China；3.Department of Physiology, Basic Medical College, Shanghai Jiaotong University, Shanghai 200025, China
  • Online:2011-06-28 Published:2011-06-27

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摘要：

目的 观察选择性阻断cGMP依赖性蛋白激酶（PKG）对恢复内毒素（LPS）孵育的大鼠血管环收缩功能的影响。方法 取SD大鼠胸主动脉血管环，随机分为正常对照组(生理盐水)、LPS组(75 μg/mL LPS)、N-硝基精氨酸甲酯（L-NAME）组(75 μg/mL LPS+0.1 mmol/L L-NAME)和DT-2三氟醋酸盐（DT-2）组(75 μg/mL LPS+0.002 mmol/L DT-2)。在体外测定LPS孵育2、3、4 h后各组血管环在苯肾上腺素（PE）作用下收缩的浓度效应曲线、最大收缩力（Emax）和产生最大收缩力的半数有效浓度（EC50）。结果 LPS孵育后各时间点血管环的收缩浓度效应曲线与对照组比较明显降低（P<0.05）。L-NAME可以提高LPS孵育2、3、4 h时血管环的收缩功能，Emax和EC50较LPS组差异有统计学意义（P<0.05）。DT2可以显著提高LPS孵育3 h时血管环的收缩功能（P<0.01），Emax和EC50的差异具有统计学意义（P<0.01），但在2 h和4 h时，DT-2组与LPS组血管环收缩的浓度效应曲线比较差异无统计学意义（P＞0.05）。结论 选择性阻断cGMP依赖性蛋白激酶可以部分恢复LPS孵育的大鼠胸主动脉血管环的收缩功能。

关键词: 内毒素, 血管环, cGMP依赖性蛋白激酶

Abstract:

Objective To investigate the effects of selective blockade of cGMP-dependent protein kinase (PKG) on contractility of vascular rings incubated with lipopolysaccharide (LPS) in rats. Methods Thoracic aortic rings of SD rats were obtained, and were randomly divided into control group, LPS group, Nω-Nitro-L-arginine methyl ester (L-NAME) group and DT-2 trifluoroacetate salt group (DT-2 group). The contraction concentration-response curve, maximal tension (Emax) and concentration required for half maximal response (EC50) of vascular rings to phenylephrine (PE) were measured in vitro at different time points (2 h, 3 h and 4 h after incubation with LPS) in each group. Results The contraction concentrationresponse curves of vascular rings after incubation with LPS for different time were significantly lower than those of control group (P<0.05). L-NAME effectively improved the contractility of vascular rings after incubation with LPS for 2 h, 3 h and 4 h, and EC50 and Emax were significantly different from those of LPS group (P<0.05). DT-2 significantly enhanced the contractility of vascular rings after incubation with LPS for 3 h (P<0.01), and EC50 and Emax were significantly different from those of LPS group (P<0.01), while there was no significant difference in contraction concentration-response curves between DT-2 group and LPS group at the time points of 2 h and 4 h （P＞0.05）. Conclusion Selective blockade of PKG can partially recover the contractility of thoracic aortic rings incubated with LPS in rats.

Key words: lipopolysaccharide, vascular rings, cGMP-dependent protein kinases