上海交通大学学报（医学版）

›› 2011, Vol. 31 ›› Issue (7): 918-.doi: 10.3969/j.issn.1674-8115.2011.07.011

• 论著（基础研究） • 上一篇    下一篇

飞秒激光切割大鼠膀胱上皮组织的体外实验

潘玉龙, 陈 方, 康 健, 梁军号   

  1. 上海交通大学 医学院附属新华医院泌尿外科, 上海 200092
  • 出版日期:2011-07-28 发布日期:2011-07-27
  • 通讯作者: 陈 方, 电子信箱: chenfang007@hotmail.com。
  • 作者简介:潘玉龙（1984—）, 男, 住院医师, 硕士生；电子信箱: yulong112358@163.com。
  • 基金资助:

    国家自然科学基金(30801154/c160606)

In vitro study of ablation of rat bladder epithelial tissues by femtosecond laser

PAN Yu-long, CHEN Fang, KANAG Jian, LIANG Jun-hao   

  1. Department of Urology, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200092, China
  • Online:2011-07-28 Published:2011-07-27
  • Supported by:

    National Natural Science Foundation of China, 30801154/c160606

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摘要：

目的 观察飞秒激光切割大鼠膀胱上皮组织的效率和副损伤范围。方法 采用不同功率(1倍、2倍、5倍、10倍和20倍阈值功率)和等效频率的飞秒激光切割SD大鼠膀胱上皮组织，切割后组织制作HE染色切片，光学显微镜下观察飞秒激光切割膀胱上皮组织的深度和副损伤范围，计算切割效率。结果 当飞秒激光功率为5～20倍阈值功率、等效频率为62.5～1 000 Hz时，对SD大鼠膀胱上皮组织的切割效率为10～120 μm/s，副损伤范围为10～100 μm。结论 飞秒激光可精细切割大鼠膀胱上皮组织，且副损伤范围小，是治疗尿路狭窄的潜在替代工具。

关键词: 飞秒激光, 等离子介导切割, 尿路狭窄, 腔内泌尿外科

Abstract:

Objective To observe the efficiency and range of collateral tissue damage in ablation of rat bladder epithelial tissues by femtosecond laser. Methods Bladder epithelial tissues of SD rats were ablated by femtosecond laser with different working powers (1, 2, 5, 10 and 20 times of threshold power and equivalent frequencies). Sections with HE staining were prepared for the ablated tissues. The depth and range of collateral tissue damage of bladder epithelial tissues ablated by femtosecond laser were observed by light microscopy, and the ablation efficiency was calculated. Results When the working powers of femtosecond laser were 5 to 20 times of threshold power and the equivalent frequencies were 62.5 Hz to 1 000 Hz, the ablation efficiency varied from 10 to 120 μm/s and the collateral tissue damage ranged from 10 to 100 μm. Conclusion Femtosecond laser can precisely ablate the rat bladder epithelial tissues with minimal range of collateral tissue damage, and may be a potential alternative for treatment of urinary stricture.

Key words: femtosecond laser, plasma-mediated tissue ablation, urinary stricture, endourology