上海交通大学学报（医学版）

›› 2012, Vol. 32 ›› Issue (12): 1587-.doi: 10.3969/j.issn.1674-8115.2012.12.015

• 论著（基础研究） • 上一篇    下一篇

丙烯酰胺对大鼠睾丸的毒性作用及其对增殖细胞核抗原蛋白表达的影响

袁玲燕, 詹 平   

  1. 重庆医科大学公共卫生与管理学院劳动卫生与环境卫生学教研室, 重庆 400016
  • 出版日期:2012-12-28 发布日期:2012-12-31
  • 通讯作者: 詹 平, 电子信箱: zanpingcqums@163.com。
  • 作者简介:袁玲燕（1987—）, 女, 硕士生；电子信箱: yuanlingyan1987@126.com。

Effects of acrylamide on reproductive toxicity and expression of proliferating cell nuclear antigen in male rats

YUAN Ling-yan, ZHAN Ping   

  1. Department of Occupational and Environmental Health, School of Public Health and Health Management, Chongqing Medical University, Chongqing 400016, China
  • Online:2012-12-28 Published:2012-12-31

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摘要：

目的 观察丙烯酰胺（AA）对大鼠睾丸的毒性作用及其对增殖细胞核抗原（PCNA）蛋白表达的影响。方法 将40只雄性SD大鼠随机分为对照组（双蒸水）、4 mg/kg AA染毒组、12 mg/kg AA染毒组和36 mg/kg AA染毒组。连续灌胃30 d后，取睾丸行苏木精-伊红染色，观察睾丸组织形态学变化；TUNEL法检测睾丸细胞凋亡情况；化学发光法检测血清中睾酮和雌二醇含量；反转录聚合酶链反应（RT-PCR）检测睾丸组织PCNA mRNA表达；免疫组织化学法和Western blotting技术检测睾丸组织PCNA蛋白表达。结果 12 mg/kg和36 mg/kg AA染毒组大鼠体质量明显低于对照组（P<0.05）。12 mg/kg和36 mg/kg AA染毒组大鼠睾丸组织生精细胞减少，生精上皮萎缩，管腔出现空泡，凋亡细胞显著增多。各染毒组大鼠血清睾酮水平均显著低于对照组（P<0.05）；12 mg/kg和36 mg/kg AA染毒组大鼠血清雌二醇水平显著低于对照组（P<0.05）。与对照组比较，12 mg/kg和36 mg/kg AA染毒组大鼠睾丸组织PCNA mRNA和蛋白表达均明显降低（P<0.05）。结论 AA染毒可引起雄性大鼠性激素分泌减少，睾丸组织发生病理学改变，凋亡细胞增多，PCNA蛋白表达减少，打乱生精细胞增殖与凋亡的动态平衡，导致生殖系统发生不同程度的功能障碍。

关键词: 丙烯酰胺, 增殖细胞核抗原, 凋亡, 睾酮, 雌二醇

Abstract:

Objective To investigate the effects of acrylamide (AA) on reproductive toxicity and expression of proliferating cell nuclear antigen (PCNA) in male rats. Methods Forty male SD rats were randomly divided into control group (double distilled water), 4 mg/kg AA treatment group, 12 mg/kg AA treatment group and 36 mg/kg AA treatment group. Thirty days after intragastric administration, testes of rats were obtained, and the histological changes of testicular tissues were observed with HE staining. The apoptosis of testicular cells was detected by TUNEL assay. The serum concentrations of testosterone and estradiol were measured by chemiluminescent method. The expression of PCNA mRNA in testicular tissues was determined by RT-PCR, and the expression of PCNA protein in testicular tissues was examined by immunohistochemistry and Western blotting. Results The body weight of rats in 12 mg/kg AA treatment group or 36 mg/kg AA treatment group was significantly lower than that in control group (P<0.05). For 12 mg/kg AA treatment group and 36 mg/kg AA treatment group, the numbers of spermatogenic cells in testicular tissues were smaller, and there were atrophy of spermatogenic epithelia, vacuoles in lumina, and increased numbers of apoptotic cells. The serum concentration of testosterone in each treatment group was significantly lower than that in control group (P<0.05), and the serum concentrations of estradiol in 12 mg/kg AA treatment group and 36 mg/kg AA treatment group were significantly lower than that in control group (P<0.05). The expression of PCNA mRNA and protein in testicular tissues in 12 mg/kg AA treatment group and 36 mg/kg AA treatment group was significantly lower than that in control group (P<0.05). Conclusion Exposure to AA may result in the decrease of sex hormone secretion, pathological changes of testicular tissues, increase of apoptotic cells and decrease of expression of PCNA protein, which may interrupt the balance between proliferation and apoptosis of spermatogenic cells and lead to the functional impairment of reproductive system.

Key words: acrylamide, proliferating cell nuclear antigen, apoptosis, testosterone, estradiol