上海交通大学学报（医学版）

›› 2013, Vol. 33 ›› Issue (2): 168-.doi: 10.3969/j.issn.1674-8115.2013.02.008

• 论著（临床研究） • 上一篇    下一篇

牙龈卟啉单胞菌临床分离株的生化反应检测与分析

周彦玢, 刘大力, 李云鹏, 束 蓉   

  1. 上海交通大学 医学院附属第九人民医院口腔医学院 牙周病科 上海市口腔医学重点实验室, 上海 200011
  • 出版日期:2013-02-28 发布日期:2013-03-07
  • 通讯作者: 束 蓉, 电子信箱: shurong123@hotmail.com。
  • 作者简介:周彦玢（1986—）, 女, 博士生；电子信箱: kathleen12@163.com。
  • 基金资助:

    国家自然科学基金（30901672）；高等学校博士学科点专项科研基金（20090073120096）；上海交通大学医学院博士创新基金（BXJ201230）；上海市教委优秀青年教师基金(jdy09048)

Analysis of biochemical reactions of Porphyromonas gingivalis clinical isolates

ZHOU Yan-bin, LIU Da-li, LI Yun-peng, SHU Rong   

  1. Department of Periodontology, the Ninth People´s Hospital, College of Stomatology, Shanghai Jiaotong University School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai 200011, China
  • Online:2013-02-28 Published:2013-03-07
  • Supported by:

    National Natural Science Foundation of China, 30901672；Research Foundation for the Doctoral Program of Higher Education of China, 20090073120096；Doctoral Innovation and Creativity Funds of Shanghai Jiaotong University School of Medicine, BXJ201230；Shanghai Scientific Special Funds for Cultivation and Selection of Excellent Young Teachers, jdy09048

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摘要：

目的 以模式菌株作为对照，对口腔中牙龈卟啉单胞菌（P.gingivalis）临床菌株进行生化反应检测，初步探讨其糖代谢性质。方法 利用API 20A和ATB Rapid ID 32A生化反应检测系统，以模式菌株W83和ATCC 33277为对照，对5株P.gingivalis临床分离菌株SJD2、SJD4、SJD5、SJD11、SJD12进行生化代谢反应检测和分析。结果 5株P.gingivalis临床菌株均具有β-半乳糖苷酶和β-N-乙酰氨基葡萄糖苷酶活性；具有水解多种氨基酸残基的能力，并能水解游离的色氨酸和精氨酸；各菌株的生化反应存在异质性。API 20A与ATB Rapid ID 32A生化反应板条的检测结果存在一定差异。结论 P.gingivalis可以多肽或氨基酸作为主要碳源或氮源，但存在部分糖代谢的能力；各菌株的生化代谢反应存在差异。ATB Rapid ID 32A可能更适合应用于龈下菌斑中革兰阴性厌氧菌的生化反应检测和鉴定。

关键词: 牙龈卟啉单胞菌, 临床菌株, 糖代谢反应

Abstract:

Objective To detect the biochemical reactions and analyze the carbohydrate metabolism of Porphyromonas gingivalis (P.gingivalis) clinical isolates, with type strains as controls. Methods API 20A and ATB Rapid ID 32A biochemical reaction testing systems were used to determine the biochemical metabolic properties of P.gingivalis clinical isolates (SJD2, SJD4, SJD5, SJD11 and SJD12), with type strains (W83 and ATCC 33277) as controls. Results All tested P.gingivalis strains possessed enzymatic activity of β-galactosidase and β-N-Acetyl-glucosaminidase. These strains had capacities to hydrolyse several amino acid residues and free amino acids such as tryptophan and arginine. In addition, there existed heterogeneity in the biochemical reactions among P.gingivalis strains. Furthermore, different bacterial identification results were found between API 20A biochemical reaction testing system and ATB Rapid ID 32A biochemical reaction testing system. Conclusion P.gingivalis may utilize peptides and amino acids as main carbon or nitrogen resources, with the ability of metabolizing carbohydrate. There are differences in biochemical properties among different strains. ATB Rapid ID 32A test system may be more suitable for identification of gram-negative subgingival anaerobes.

Key words: Porphyromonas gingivalis, clinical isolates, carbohydrate metabolism