HPV16 E2对宫颈癌SiHa细胞上皮间质转化的影响
网络出版日期: 2013-09-16
基金资助
上海市科委项目(09411962500);上海市科委浦江人才计划D类项目(12PJD002);国家自然基金青年项目(81201541);卫生部行业基金项目(201002013)
Influence of HPV16 E2 on epithelial-mesenchymal transition of cervical cancer SiHa cells
Online published: 2013-09-16
Supported by
Shanghai Science and Technology Committee Foundation, 09411962500, 12PJD002; National Natural Science Foundation of China, 81201541; Foundation of the Ministry of Health of China, 201002013
目的 探讨HPV16 E2对宫颈癌SiHa细胞上皮间充质细胞转化的影响。方法 采用脂质体转染p-CAG-MYC-HPV16 E2至宫颈癌SiHa细胞中,倒置相差显微镜下观察转染前后细胞形态的改变;RT-PCR及Western blotting检测转染前后Twist2、上皮细胞标志物E-cadherin及间质细胞标志物Vimentin表达的变化。结果 成功转染HPV16 E2至宫颈癌SiHa细胞中。转染后,上皮细胞由椭圆形变成梭形,细胞间隙增大;Twist2和Vimentin表达上调,E-cadherin表达下调。结论 HPV16 E2能在体外诱导宫颈癌细胞发生上皮间充质细胞转化,促进早期癌变。
钱文艳 , 张稼闻 , 董 煜 , 等 . HPV16 E2对宫颈癌SiHa细胞上皮间质转化的影响[J]. 上海交通大学学报(医学版), 2013 , 33(8) : 1074 . DOI: 10.3969/j.issn.1674-8115.2013.08.007
Objective To investigate the influence of HPV16 E2 on epithelial-mesenchymal transition of cervical cancer SiHa cells. Methods Lipofectamine was used to transfect p-CAG-MYC-HPV16 E2 gene into cervical cancer SiHa cells. The morphology of cells was observed before and after transfection with phase-contrast microscope, and the expression of Twist2, E-cadherin and Vimentin before and after transfection was determined by RT-PCR and Western blotting. Results HPV16 E2 was successfully transfected into SiHa cells. After transfection, the epithelial cells became spindle-shaped, with enlarged intercellular space, and the expression of Twist2 and Vimentin was up-regulated, while the expression of E-cadherin was down-regulated. Conclusion HPV16 E2 can induce epithelialmesenchymal transition of cervical cancer cells, and promote cervical carcinogenesis.
Key words: HPV16 E2; epithelial-mesenchymal transition; transfection; cervical cancer
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