目的 探讨microRNA-128a （miR-128a）在非小细胞肺癌（NSCLC）组织中的表达及其对NSCLC细胞增殖和迁移的影响。方法 用Real-Time PCR检测miR-128a在NSCLC与癌旁正常组织中的表达；用miR-128a mimics转染NSCLC细胞系A549，miR-128a inhibitor转染NSCLC细胞系H1299，通过细胞增殖分析（MTT assay）、细胞迁移分析（Transwell assay）和软琼脂克隆形成分析（soft-agar assay）检测miR-128a对NSCLC细胞增殖、迁移以及体外克隆形成能力的影响。结果 miR-128a在NSCLC组织中表达较癌旁正常组织明显下调；miR-128a高表达可抑制A549细胞的增殖、迁移和克隆形成；而抑制miR-128a的表达会促进H1299细胞的增殖、迁移和克隆形成。结论 NSCLC组织中miR-128a表达下调，在NSCLC发生和发展中发挥重要作用。

Objective To investigate the expression of microRNA-128a (miR-128a) in non-small cell lung cancer （NSCLC） tissues and its effects on proliferation and migration of NSCLC cells. Methods The miR-128a expression in NSCLC and normal lung tissues was determined by Real-Time PCR. The effects of miR-128a on proliferation and migration of NSCLC cells were tested by MTT assay, Transwell assay, and soft-agar assay with A549 cells transfected with miR-128a mimics and H1299 cells transfected with miR-128a inhibitor. Results The expression level of miR-128a was significantly down-regulated in NSCLC tissues than that in normal lung tissues. Overexpression of miR-128a could inhibit proliferation, migration, and colony formation of A549 cells. And down-regulation of miR-128a in H1299 cells could promote proliferation, migration, and colony formation. Conclusion The expression of miR-128a is down-regulated in NSCLC tissues, and miR-128a plays an important role in the initiation and development of NSCLC.