上海交通大学学报(医学版)

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microRNA-128a对非小细胞肺癌细胞增殖和迁移的影响

王 琳,娄加陶   

  1. 上海交通大学附属胸科医院检验科, 上海 200030
  • 出版日期:2014-03-28 发布日期:2014-04-02
  • 通讯作者: 娄加陶, 电子信箱: loujiatao@126.com。
  • 作者简介:王 琳(1988—), 女, 硕士生; 电子信箱: linwangssmu@163.com。
  • 基金资助:

    国家自然科学基金资助项目(81372193);上海市科委基础研究项目(13JC1405502)

Effects of microRNA-128a on proliferation and migration of non-small cell lung cancer cells

WANG Lin, LOU Jia-tao   

  1. Department of Laboratory, Shanghai Chest Hospital, Shanghai Jiao Tong University, Shanghai 200030, China
  • Online:2014-03-28 Published:2014-04-02
  • Supported by:

    National Natural Science Foundation of China, 81372193; Basic Research Project of Science and Technology Commission of Shanghai Municipality, 13JC1405502

摘要:

目的 探讨microRNA-128a (miR-128a)在非小细胞肺癌(NSCLC)组织中的表达及其对NSCLC细胞增殖和迁移的影响。方法 用Real-Time PCR检测miR-128a在NSCLC与癌旁正常组织中的表达;用miR-128a mimics转染NSCLC细胞系A549,miR-128a inhibitor转染NSCLC细胞系H1299,通过细胞增殖分析(MTT assay)、细胞迁移分析(Transwell assay)和软琼脂克隆形成分析(soft-agar assay)检测miR-128a对NSCLC细胞增殖、迁移以及体外克隆形成能力的影响。结果 miR-128a在NSCLC组织中表达较癌旁正常组织明显下调;miR-128a高表达可抑制A549细胞的增殖、迁移和克隆形成;而抑制miR-128a的表达会促进H1299细胞的增殖、迁移和克隆形成。结论 NSCLC组织中miR-128a表达下调,在NSCLC发生和发展中发挥重要作用。

关键词: 非小细胞肺癌, microRNA, miR-128a

Abstract:

Objective To investigate the expression of microRNA-128a (miR-128a) in non-small cell lung cancer (NSCLC) tissues and its effects on proliferation and migration of NSCLC cells. Methods The miR-128a expression in NSCLC and normal lung tissues was determined by Real-Time PCR. The effects of miR-128a on proliferation and migration of NSCLC cells were tested by MTT assay, Transwell assay, and soft-agar assay with A549 cells transfected with miR-128a mimics and H1299 cells transfected with miR-128a inhibitor. Results The expression level of miR-128a was significantly down-regulated in NSCLC tissues than that in normal lung tissues. Overexpression of miR-128a could inhibit proliferation, migration, and colony formation of A549 cells. And down-regulation of miR-128a in H1299 cells could promote proliferation, migration, and colony formation. Conclusion The expression of miR-128a is down-regulated in NSCLC tissues, and miR-128a plays an important role in the initiation and development of NSCLC.

Key words: non-small cell lung cancer, microRNA, miR-128a