子宫内膜异位症不孕与CD4+CD25+FoxP3调节性T淋巴细胞的相关性研究
网络出版日期: 2014-08-11
基金资助
上海市卫生局青年科学研究项目(2010Y1)
Relationship between CD4+CD25+FoxP3 regulatory T cells and endometriosis associated infertility
Online published: 2014-08-11
Supported by
Foundation for Young Scientists of Shanghai Municipal Health Bureau, 2010Y1
目的 探讨子宫内膜异位症(EM)不孕与围种植期外周血及在位内膜CD4+CD25+FoxP3调节性T淋巴细胞(Tregs)变化的相关性。方法 采用流式细胞术检测27例不同期别EM不孕患者外周血单个核细胞CD4+CD25+FoxP3 Tregs数量及其占CD4+T淋巴细胞的百分比,RT-PCR检测在位内膜CD4+CD25+FoxP3 mRNA表达水平。以已生育非EM女性作为对照组(n=20)。结果 与对照组比较,轻度EM不孕组外周血单个核细胞中CD4+CD25+FoxP3 Tregs数量显著增多,其占CD4+T淋巴细胞百分比增高,差异均有统计学意义(P<0.05);重度EM不孕组CD4+CD25+FoxP3 Tregs数量及其占CD4+T淋巴细胞百分比均显著高于轻度EM不孕组,差异均有统计学意义(P<0.05)。在位内膜FoxP3 mRNA相对表达量检测结果显示,轻度EM不孕组显著高于对照组 (P=0.000 1),重度EM组显著高于轻度EM组和对照组(P<0.000 1)。结论 EM不孕围种植期CD4+CD25+FoxP3 Tregs与内膜容受性异常导致不孕的机制无直接关系;CD4+CD25+FoxP3 Tregs可能与EM的发生和发展有关,可能加重EM所致病变而导致不孕。
关键词: 子宫内膜异位症; CD4+CD25+FoxP3; 不孕症
梁 艳 , 陈淑芳 , 张 健 . 子宫内膜异位症不孕与CD4+CD25+FoxP3调节性T淋巴细胞的相关性研究[J]. 上海交通大学学报(医学版), 2014 , 34(7) : 997 . DOI: 10.3969/j.issn.1674-8115.2014.07.008
Objective To explore the relationship between the endometriosis (EM) associated infertility and changes of CD4+CD25+FoxP3 Tregs in the peripheral blood and eutopic endometrium during peri-implantation phase. Methods Twenty-seven women with EM associated infertility of different stages were selected. The CD4+CD25+FoxP3 Tregs counts in peripheral blood mononulear cells (PBMCs) and the ratio of CD4+T cells and PBMCs were detected by the flow cytometry. The expression of CD4+CD25+FoxP3 mRNA of eutopic endometrium was detected by the RT-PCR. The controls were twenty procreated women without EM (n=20). Results Compared to the controls, the CD4+CD25+FoxP3 Tregs counts in PBMCs and the ratio of CD4+ T cells and PBMCs of women with minor EM were significantly higher. The differences were statistically significant (P<0.05). The CD4+CD25+FoxP3 Tregs counts in PBMCs and the ratio of CD4+ T cells and PBMCs of women with severe EM were significantly higher than those of women with minor EM. The differences were statistically significant (P<0.05). The relative expression of FoxP3 mRNA of eutopic endometrium showed that the expression of women with minor EM was significantly higher than that of controls (P=0.000 1), and the expression of women with severe EM was significantly higher than that of controls and women with minor EM (P<0.000 1). Conclusion The CD4+CD25+FoxP3 Tregs in the peri-implantation phase are not directly relevant to the pathogenesis of abnormal endometrial receptivity of infertile women with EM. CD4+CD25+ FoxP3 Tregs may be relevant to the incidence and development of EM and may aggravate lesions caused by EM and lead to the infertility.
Key words: endometriosis; CD4+CD25+ FoxP3; infertility
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