目的 观察糖化终末产物（AGEs）对人结肠癌HCT116细胞氧化应激的影响。方法 运用活性氧（ROS）捕获剂孵育HCT116细胞，通过流式细胞仪和荧光显微镜检测AGEs刺激后HCT116细胞内ROS水平；同时用N-乙酰半胱氨酸（NAC）干预氧化应激状态，观察是否影响AGEs诱导的ROS增加及细胞增殖。结果 AGEs刺激后HCT116细胞中ROS的生成增加。HCT116细胞经NAC处理后，AGEs刺激所致ROS生成增加和促细胞增殖能力都被明显抑制，差异有统计学意义（P<0.05）。结论AGEs通过激活氧化应激反应促进人结肠癌HCT116细胞的增殖。

Objective To observe the effects of advanced glycation end products (AGEs) on the oxidative stress of HCT116 colorectal cancer cells. Methods HCT116 cells were cultured by the reactive oxygen species (ROS) capture agent. The ROS level of AGEs-treated HCT116 cells was detected by the flow cytometry and fluorescent microscopy. The oxidative stress status was intervened by the N-acetyl cysteine (NAC) and its effects on ROS increase and cell proliferation induced by AGEs were observed. Results ROS level increased after HCT116 cells were treated by AGEs. ROS increase and cell proliferation induced by AGEs were significantly inhibited after HCT116 cells were treated by NAC. The differences were statistically significant (P<0.05). Conclusion AGEs enhances the proliferation of HCT116 colorectal cancer cells by activating the oxidative stress response.