网络出版日期: 2016-01-13
基金资助
国家自然科学基金(81200877)
Preparation of an urine internal quality control substance for fluorescence PCR detection of cytomegalovirus DNA
Online published: 2016-01-13
Supported by
National Natural Science Foundation of China, 81200877
目的 制备人巨细胞病毒核酸定量荧光PCR检测的尿液室内质控物,并观察其稳定性。方法 收集荧光PCR检测结果为阳性的尿液标本,经简单过滤处理,-80 ℃保存观察其稳定性;并比较-20 ℃与-80 ℃保存条件对质控物稳定性的影响。结果 在120次检测结果中,前20次采用即刻法,室内质控结果均在控;20次后绘制Levey-Jennings质控图,根据Westgard多规则评价室内质控物,结果均在控;-20 ℃与-80 ℃保存条件对制备的室内质控物稳定性无影响。结论 简单方法制备的尿液室内质控物具有较好的稳定性,可以用作人巨细胞病毒核酸定量荧光PCR检测的室内质控物。
陈克平 , 徐玉蓉 , 李丽 . 人巨细胞病毒核酸定量荧光PCR检测的尿液室内质控物制备[J]. 上海交通大学学报(医学版), 2015 , 35(11) : 1766 . DOI: 10.3969/j.issn.1674-8115.2015.11.034
Objective To prepare an urine internal quality control substance for fluorescence PCR detection of cytomegalovirus DNA and observe the stability. Methods Urine samples with positive CMV-DNA were collected, filtered, and preserved at -80℃ to observe its stability. The effect of preservation conditions of -80 ℃ and -20 ℃ on the stability of quality control substance was compared. Results Among 120 tests, first 20 tests adopted the instance method and the results were under control. Levey-Jennings quality chart was plotted after 20 tests and the results were all under control according to Westgard rules. The preservation conditions of -80 ℃ and -20 ℃ did not affect the stability of prepared internal quality control substance. Conclusion The urine internal quality control substance prepared by simple method is stable and can be used as internal quality control substance for fluorescence PCR detection of cytomegalovirus DNA.
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