目的 ·针对泛耐药肺炎克雷伯菌寻找宽宿主谱噬菌体，并对其生物学特性、基因组成进行分析。方法 ·以泛耐药肺炎克雷伯菌为宿主菌，从医院污水中分离纯化得到针对宿主菌的泛耐药肺炎克雷伯菌噬菌体。电子显微镜观察噬菌体的大小和形态，分析其效价、宿主谱、酸碱稳定性和热稳定性。抽取噬菌体DNA进行测序及分析。结果 ·分离到l株肺炎克雷伯菌噬菌体，命名为SH-Kp152234。电子显微镜观察显示其属短尾噬菌体，基因组为线状双链DNA，全长为40 578 bp，GC含量为52.85％。预测共有49个开放阅读框，保守序列具有已知的相关功能。结论 ·筛选得到肺炎克雷伯菌噬菌体SH-Kp152234的宿主谱较宽，潜伏期较短，可适应不同的温度和pH值，为治疗泛耐药肺炎克雷伯菌感染提供潜在依据。

Objective · To identify broad-spectrum bacteriophages against extensively drug-resistant Klebsiella pneumonia and analyze their characteristics by biological and genomic methods. Methods · Multi-drug resistant Klebsiella pneumonia strains collected from a hospital were used as host bacteria to isolate and purify broad-spectrum phages in the wastewater at the same hospital area. The size and shape of phages were observed by transmission electron microscope. Titer, host range, pH stability and thermal stability were measured. Moreover, the DNA extracted from the phage SH-Kp152234 was sequenced and analyzed. Results · One strain of bacteriophage against Klebsiella pneumonia was isolated and named as SH-Kp152234. The electron microscope revealed it belongs to Podoviridae family. Moreover, genome of SH-Kp152234 showed to be a linear double-stranded DNA of 40 578 bp with the GC content of 52.85%. It was predicted to have 49 open reading frames with related known functions. Conclusion · SH-Kp152234, with a broad host range and a short latent period, which could exert its activity in a wide range of temperature and pH, is a promising candidate to be exploited in the treatment of multiple drug-resistant Klebsiella pneumonia.