目的 ·运用斑马鱼模型初步探讨胚胎期双酚 A（bisphenol A，BPA）暴露对斑马鱼发育和神经行为的影响。方法 ·对受精后小时数（hours post-fertilization，hpf）为 4的正常斑马鱼受精卵以不同浓度 BPA（0、2.5、25、250、2 500 μg/L）作染毒处理；观察并记录斑马鱼存活率、畸形率和孵化率，以及斑马鱼胚胎 24 hpf自主运动和 48 hpf心率，并对发育至 144 hpf的斑马鱼幼鱼进行神经行为学检测。结果 ·各浓度 BPA均未影响斑马鱼的存活率、畸形率和孵化率。暴露于 2 500 μg/L浓度 BPA的斑马鱼胚胎 24 hpf自主运动和 48 hpf心率低于对照组（ P0.000），发育至 144 hpf的幼鱼运动总距离和平均速度降低（ P0.000）。暴露于 250 μg/L和 2 500 μg/L浓度 BPA的斑马鱼幼鱼在强光刺激惊恐反射检测中运动行为改变，主要表现为在强光刺激下运动总距离减少（ P<0.05）。结论 ·胚胎期 BPA暴露对斑马鱼具有一定的发育毒性和神经毒性。

Objective · To investigate the effects of bisphenol A (BPA) on the development and neurobehavior of zebrafish. Methods · The zebrafish fertilized eggs that developed to 4 hpf (hours post-fertilization) were treated with different concentrations of BPA (0, 2.5, 25, 250, and 2 500 μg/L). Survival rates, malformation rates, hatching rates, 24 hpf autonomic movement, and 48 hpf heart rates in the groups were observed and recorded. And the neurobehavioral test of larval zebrafish that developed to 144 hpf was performed. Results · The BPA exposure did not affect the survival rates, malformation rates and hatching rates of zebrafish. Compared with the control group, the autonomic movement in 24 hpf and heart rate in 48 hpf of zebrafish embryos that exposed to 2 500 μg/L BPA decreased (P0.000), and the total distance and average speed of larval zebrafish that developed to 144 hpf also decreased (P0.000). The behavior of larval zebrafish exposed to 250 μg/L and 2 500 μg/L BPA changed on light-evoked startle escape response activity, mainly including the decrease of total distance under strong light stimulation (P<0.05). Conclusion · BPA exposure during embryonic phase has developmental toxicity and neurotoxicity on zebrafish.