目的·探索硫酸吲哚酚（indoxyl sulfate，IS）对人牙周膜细胞（human periodontal ligament cells，hPDLCs）增殖、炎症因子和活性氧（reactive oxygen species，ROS）表达的影响。方法·采用组织块培养法体外培养hPDLCs；采用四甲基偶氮唑盐比色（methyl thiazolyl tetrazolium，MTT）法观察IS（按0、62.5、125、250、500、1 000 μmol/L 分组）对hPDLCs增殖活性的影响；采用实时荧光定量PCR和ELISA检测各组炎症因子IL-1β、IL-6、IL-8 mRNA和蛋白的表达；采用2, 7-二氯荧光素二乙酸盐（2, 7-dichlorofluorescin diacetate，DCFDA）荧光探针检测各组hPDLCs胞内ROS的表达。结果·在24、48、72 h时，125 μmol/L的IS可抑制hPDLCs增殖，与对照组相比差异具有统计学意义（PIL-1β、IL-6、IL-8 mRNA和蛋白的表达，上调胞内ROS水平。结论·IS可通过抑制hPDLCs活性、促进炎症因子表达和提高胞内ROS水平，从而在慢性肾病与牙周炎的相互关联中起重要作用。

Objective · To explore the effects of Indoxyl sulfate (IS) on proliferation activity, of inflammatory factors and reactive oxygen species (ROS) in human periodontal ligament cells (hPDLCs). Methods · The primary hPDLCs were culturedusing tissue explant method in vitro. MTT assay was employed to evaluate the effect of IS on proliferation activity of hPDLCs. The mRNA and protein s of IL-1β, IL-6, and IL-8 were detectedusing real-time PCR and ELISA assay. DCFDA fluorescence probe was used to detect intracellular ROS and ROS in the cytoplasm under fluorescence microscope. Results · The viability of hPDLCs was inhibitedIS at the concentration of 125 μmol/L on 24, 48 and 72 hours. The inhibitory effect was presented in a dose- and time- dependent manner. IS could upregulate the mRNA and protein of inflammatory cytokines including IL-1β, IL-6 and IL-8 as well as promote ROS in hPDLCs. Conclusion · IS may play an important role in the association between chronic kidney diseases and periodontitis through inhibiting the activity of hPDLCs, promoting the of inflammatory cytokines and increasing intracellular ROS level.