目的·探讨胃食管结合部腺癌（adenocarcinoma of the esophagogastric junction，AEG）患者肿瘤组织中T细胞免疫球蛋白黏蛋白3（T cell immunoglobulin mucin 3，Tim-3）、半乳凝素9 （galectin 9，Gal-9）表达及CD3+、CD8+ T细胞浸润情况与AEG患者临床特征及生存预后的关系。方法·采用病例回顾性研究，收集2005年12月—2013年12月上海交通大学医学院附属仁济医院收治的116例AEG患者的临床资料及随访资料，免疫组织化学法检测Tim-3、Gal-9、CD3、CD8蛋白在肿瘤组织的表达情况，比较不同蛋白表达水平、T细胞浸润水平患者间的临床特征及预后。结果·免疫组织化学检查结果显示，Tim-3主要表达在浸润的免疫细胞，Gal-9主要表达在肿瘤细胞。患者临床特征分析结果显示，Tim-3表达水平与Siewert分型相关（P=0.030），CD8+ T细胞的浸润水平与肿瘤TNM分期相关（P=0.042）。患者生存分析结果显示，CD8+ T细胞高浸润患者拥有较好的生存预后（P=0.047），不同Tim-3和/或Gal-9蛋白表达水平、不同CD3+ T细胞浸润水平患者间预后差异无统计学意义。结论·CD8+ T细胞高浸润AEG患者通常伴有较早的TNM分期，且患者预后较好；不同Tim-3/Gal-9表达水平的AEG患者预后未发现明显差异 。

Objective · To detect T cell immunoglobulin mucin 3 (Tim-3) and galectin 9 (Gal-9) expression as well as CD3+ T cells and CD8+ T cells infiltration in the tumor tissues of adenocarcinoma of the esophagogastric junction (AEG), and analyze their correlations with the patients’ clinical characteristics and survival prognosis. Methods · A retrospective case study was used to collect clinical data and follow-up data of 116 AEG patients who were admitted to Renji Hospital, Shanghai Jiao Tong University School of Medicine from Dec. 2005 to Dec. 2013. Tim-3, Gal-9, CD3, and CD8 protein expressions were detected by immunohistochemistry in the tumor tissues, and the clinical characteristics and prognosis were compared among the patients with different levels of protein expression and T cells infiltration. Results · The results of immunohistochemistry showed that Tim-3 mainly expressed in the infiltrating immune cells, and Gal-9 mainly expressed in the tumor cells. The analysis on the clinical characteristics revealed that Tim-3 expression level was related to the Siewert classification (P=0.030) and CD8+ T cells infiltration level was related to the tumor TNM stage (P=0.042). The results of survival analysis showed that the patients with high level of CD8+ T cells infiltration had a better survival prognosis (P=0.047). However, there was no difference in the prognosis among the patients with different Tim-3 and/or Gal-9 expression levels or with different CD3+ T cell infiltration levels. Conclusion · AEG patients with high level of CD8+ T cells infiltration usually have earlier TNM stages and better prognosis. There is no significant difference in the prognosis of AEG patients with different Tim-3/Gal-9 expression levels.